Electrokinetic injection microchip capillary electrophoresis

J. Palmer, N. J. Munro, and J. P. Landers, A Universal Concept for Stacking Neutral Analytes in Micellar Capillary Electrophoresis, Anal. Chem. 1999, 71, 1679 J. Palmer, D. S. Burji, N. J. Munro, and J. P. Landers, Electrokinetic Injection for Stacking Neutral Analytes in Capillary and Microchip Electrophoresis, Anal. Chem. 2001, 73, 725 J. P. Quirino, S. Terabe, and... 

Palmer, J., Burgi, D.S., Munro, N.J., Landers, J.P., Electrokinetic injection for stacking neutral analytes in capillary and microchip electrophoresis. Anal. Chem. 

Li and coworkers described the separation of FITC and FllC-labeled antihuman IgG by zone electrophoresis in a glass microchip. The two compounds were electrokinetically injected into the separation channel for 2 s and separated in less than 12 s. A comparison between the separation performance in capillary and microchip showed that higher efficiency was achieved in the microchip format due to the shorter separation length and the higher electric field that were applied in microfluidic structures. 

Xu, Z. Q., Hirowaka, T., Nishine, T., and Arai, A., High-sensitivity capillary gel electrophoretic analysis of DNAfragments on an electrophoresis microchip using electrokinetic injection with transient isotachophoretic preconcentration. Journal of Chromatography, 990, 53-61, 2003. 

Tsai CH, Yang RJ, Tai CH, Fu LM (2005) Numerical simulation of electrokinetic injection techniques in capillary electrophoresis microchips. Electrophoresis 26 674-686... 

Keywords Capillary electrophoresis Micellar electrokinetic chromatography Microchip pTAS Nonaqueous capillary electrophoresis How injection analysis Solid-phase microextraction Bipolar electrochemical swimmers... 

Shadpour et al. [81] and Osiri et al. [82] employed SDS micro-capillary gel electrophoresis (SDS p-CGE) and micellar electrokinetic capillary (MEKC) electrophoresis in the first and second dimensions, respectively, to sort intact proteins using a poly (methylmethacrylate), PMMA, microchip. A diagram of the microchip is shown in Fig. 4. The electrophoresis commenced in the first dimension for a prescribed amount of time and, then, the bands from the first dimension were sequentially injected into the second dimension for development. The 2D electrophoresis system could generate a peak capacity of 2,600 for proteins isolated from fetal calf serum (see Fig. 4). 

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