Effect of Candidate Compounds with Antisecretory Potential on Serum Gastrin Levels

Effect of Candidate Compounds with Antisecretory Potential on Serum Gastrin Levels 

Groups of 10-15 rats weighing 90-110 g are treated daily for 10 weeks with the candidate compound (omeprazole as standard at doses of 10 or 30 mg/kg p.o.). After treatment for 2, 4, 7, and 10 weeks, blood samples are collected under ether anesthesia by retro orbital puncture. Gastrin is determined by a commercially available radioimmunoassay kit. At the end of the study of 10 weeks, the animals are studied for their gastric acid output using the pylorus ligation (Shay technique). 

Serum gastrin levels are determined as pg/ml. Statistical differences (p 0.05) are calculated using appropriate statistical methods. 

Katz et al. (1987) described a five-day test to predict the long-term effects of gastric antisecretory agents on serum gastrin in rats. 

Arnold R, Koop H, Schwarting H et al. (1986) Effect of acid inhibition on gastric endocrine cells. Scand J Gastroenterol 21 (Suppl 125) 14-19 

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