Effect of age on tissue content

In the meantime, the formation of the main alkaloids in C. ipecacuanha under a variety of conditions has been extensively investigated emetine (1) in callus cultures (49) and under the effects of L-tyrosine supplementation (5t)) emetine (1) and cephaeline (2) in Panamanian ipecac (57), in Nicaraguan ipecac (52), in regenerates obtained by clonal propagation (53,54), in tissue cultures (55) and under the effects of exogenous feeding of shikimic acid and L-phenylalanine (55), in cell suspension and excised root cultures (57), in adventitious root cultures (58), and in callus cultures (56,59) and the effects of age and electrokinetic potential (60) ipecoside (7) in the roots (61) and the effect oi Azotobacter, leaf mold, and farmyard manure on alkaloid content (62). In addition, micropropagation systems for C. ipecacuanha have been developed (63-65). 

Whereas much attention has been paid to the effects of dietary fluoride on the skeleton of domestic and experimental animals and man (T8), knowledge about the accumulation of fluoride in soft tissues such as heart, liver or kidney is scarce. The results in Table X indicate that the fluoride content of the kidneys of mice increased with age when the dietary Ca was low and fluoride feeding resulted in much higher levels at 653 days of age irrespective of the dietary Ca content. It is likely that such high fluoride levels had an adverse effect on kidney function. In cases of chronic fluoride intoxication, kidney function was reported to be impaired in the majority of cases (18). It should be noted that fluoride accumulated in the kidneys of mice even though the capacity of bone to store fluoride (5000 yg/g dry weight) without skeletal damage was not reached. 

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