TEAE cellulose

CM cellulose (carboxymethyl) CM 22, 23 cellulose P cellulose (phosphate) SE cellulose (sulphoethyl) SM cellulose (sulphomethyl) DEAE cellulose (diethylaminoethyl) DE 22, 23 cellulose PAB cellulose (p-aminobenzyl) TEAE cellulose (triethylaminoethyl) ECTEOLA cellulose... 

DEAE cellulose (diethylaminoethyl) DE 22, 23 cellulose PAB cellulose (p-aminobenzyl) TEAE cellulose (triethylaminoethyl) ECTEOLA cellulose... 

In this laboratory, attempts (G6, G8) have been made to purify and crystallize human placental alkaline phosphatase enzyme by a number of procedures involving homogenization with 0.05 M Tris buffer (pH 8.6), extraction with butanol, ammonium sulfate precipitation, exposure to heat, ammonium sulfate fractionation, dialysis, repeated ethanol fractionation, gel filtration with Sephadex G-200 (Fig. 18), continuous curtain electrophoresis on paper (Beckman Model CP), multiple TEAE-cellulose anion exchange chromatography, and equilibrium dialysis. Variant A (electrophoretically fast-moving) of human placental alkaline... 

It has been shown that FeMoco can be extracted into many organic solvents,provided proper combinations of cations and anions are present in the solvent. The role of the cation is to balance the charge of the negatively charged cofactor. The role of the anion is to displace the cofactor from anion-exchange columns, such as DEAE cellulose or TEAE cellulose, to which the cofactor and/or its protein source had been adsorbed. The ability to dissolve cofactor in such solvents as CH3CN, acetone, THF, and even benzene should facilitate attempts at further characterization and crystallization. ... 

DEAE-cellulose in the borate form (instead of the usual acetate form) and TEAE-cellulose in the hydroxyl form are useful alternatives. The former is useful for the isolation of phosphatidylethanolamine and the latter is also valuable for the preparation of acidic lipids (Christie, 1982). Columns should be prepared as described by Rouser et ah, (1967). 

Fluharty et aL (1974) have reported the isolation of S-labelled cerebroside sulphate. They injected the brains of young rats intracerebrally with [ S]-sulphate and sacrificed the animals 3 days later. Total lipids were extracted and glycerolipids destroyed by alkaline hydrolysis. TEAE-cellulose column chromatography was then used to purify the [ Sjsulphatide. Jatzkewitz and Nowoczek (1967) have synthesized o-galactose 3-sulphate and shown it to be identical to the galactose sulphate present in brain sulphatides. 

Chromatography on TEAE-cellulose of this material resulted in a product with about 200-250 Ivy dog units/mg of cholecystokinin and a corresponding increase in the pancreozymin activity (Jorpes and Mutt 1961 a, 1962). 

Pancreozymin. As pointed out by the present authors in 1961 and 1962, it is quite remarkable how closely cholecystokinin and pancreozymin accompany each other during the various purification steps, whereas secretin separates very early. This applies to the chromatography on the acidic carboxymethyl cellulose and on the basic TEAE-cellulose up to a strength of 200-250 Ivy dog units of CCK per mg substance. 

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