ECTEOLA-Cellulose Layers for Sugar Phosphates

A suspension of sieved ECTEOLA-cellulose powder (Firm 127) (2 g) in 0.004 M ethylenediaminetetraacetic acid, pH 7.0 (18 ml) is shaken vigorously for 5 min. The slurry is spread uniformly on a glass plate (20 X 20 cm) and dried overnight at room temperature giving a layer about 200 [X thick. The dried layer is then sprayed with 0.1 M ammonium tetraborate, pH 9.0, hnd dried at 50° for 30 min. No changes are found on storing the plates for 3 days. 

Calcium suKate hemihydrate (0.8 g) is ground for 1 min in a mortar with distilled water (5 ml). The Cehte adsorbent (Filter Cel or Hyflo Super-Cel) (15 g) and additional water (60 ml) are added and the mixture is ground for 1—2 min. The smooth slurry is spread with an applicator in the usual way to give five 8 x 8 in (20 x 20 cm) plates which are air-dried for 10—15 min and then dried at 100° for 30 min. 

A suspension of 200-mesh Celite 535 (18 g) in 0.25 N sodium hydroxide is mixed with a suspension of powdered potato starch (1.5 g) in water 

Sugar Anisaldehyde-suKuric acid (No. 11) [1] Naphthol-resorcinol-sulfuric acid (No. 175) [8] Aniline-diphenylamine (No. 8) [7] 

D-Xylose grey light blue bright blue 

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