ECLIA

Based on many of the advances described above in electrochemical approaches to immunoassay, it is tempting to conclude that commercialization of some of the approaches is imminent. This may be true, but the historical use of optical methods for many clinical chemistry tests coupled with their rapidly growing use in immunoassay is a difficult barrier for any radically different method to overcome, though electrochemical sensors have become more important in the clinical chemistry laboratory over the last decade. In any event, to be successful ECIA methods will have to demonstrate clear superiority over existing and emerging technologies in both cost and performance. Some of the more recently described approaches such as those using enzyme amplified amperometric detection and ecLIA appear... 

An example for an anti-HBe test with a direct label is the electrochemiluminescence immunoassay ECLIA from Roche Diagnostics (automated on Elecsys immunoassay analyser), whereas the Im-mulite anti-HBe test from Diagnostics Products Corp. represents an enzyme-amplified chemiluminescence immunoassay with sustained signal (automated on the Immulite chemiluminescent system). 

ECL Electrochemiluminescence, electrogenerated CL ECLIA Electrochemiluminoimmunoassay EL Electrogenerated luminescence... 

In analytical chemistry, ECL can be used for determination of electrochemilu-minescent compotmds, or other more interesting compounds using electrochemilu-minescent labels in techniques and protocols analogous to fluoroimmunoassays or chemiluminoitnmunoassays. Electrochemiluminoimmunoassay (ECLIA) methods are not very mature yet, and their value for analytical science will certainly grow in the future. General reviews on ECL have been published quite frequently [6-11]. 

Sm were the central ions [40], At the same time, we experimentally developed the first ECLIA utilizing Tb(lll) chelate label [41], At that time, the excitation and luminescence mechanisms were still waiting to be resolved, although it already seemed possible that a solvated electron might be a mediating species in the cathodic excitation steps at oxide-covered aluminum electrodes. 

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