Dulbecco’s modified eagle medium

Figure 4 Effect on the serum stability of the incorporation of 5% cholesterol poly(ethy-lene glycol) (PEG) into cationic lipoplexes [hpopolyamine RPR209120/DOPE1/1, ratio (mol) lipid/DNA = 10 in 150 mM NaCl]. Lipoplexes were incubated in DMEM + 10% SVF, at 37°C, aliquots were regularly sampled and monitored by dynamic diffusion. Results represent a mean between three measurements. Error bars are not presented to simplify the graph, but differences among PEG, PEG-1, and PEG-2 are significant. Abbreviations PEG, poly(ethylene glycol) DOPE, dioleylphosphatidylethanolamine DMEM, Dulbecco s Modified Eagle Medium.

Appropriate culture medium for the specific tumor cell line(s) under test (e.g., Dulbecco s Modified Eagle Medium (DMEM) lx liquid GlutaMAX I, 4,500 mg/L o-glucose, sodium pyruvate DMEM/F12 (1 1) lx liquid with GlutaMAX I). 

Brandley and Schnaar [140] immobilized a synthetic nonapeptide, Tyr- Ala-Val Thr-Gly Arg-Gly-Asp-Ser, on a polyacrylamide gel, which had been prepared by a ternary copolymerization of acrylamide, bisacrylamide and the acrylic ester of. V-hydroxysuccinimide. They reported that Balb/c 3T3 mouse fibroblast cells (in Hepes-buffered Dulbecco s modified Eagle medium) adhered readily to the peptide-derivatized surfaces, even in the absence of serum, although long-term cell growth required the presence of serum. It was noticed that reference nonapeptide. Tyr-Arg-Leu-Glu-Asp-Pro-Ala-Met-Trp, which has no RGD sequence, failed to promote cell-attachment. 

Dulbecco s Modified Eagles Medium (DMEM) containing antibiotics (100 U/mL penicillin, 100 U/mL streptomycin, 0 25 pg/mL amphotericin B, 50 pg/mL genta-mycin) and 10% (v/v) fetal bovine serum (FBS). 

Dulbecco s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and TrypLE Express Stable Trypsin-Like Enzyme with Phenol Red (Invitrogen, Carlsbad, CA). 

HeLa cell cultures are propagated in Dulbecco s Modified Eagle Medium (D-MEM) (500 mL) (Gibco) supplemented with 10% fetal bovine semm (FBS, Bio West), 5 mL MEM non-essential amino acids (100 x ) (Gibco), 5 mL sodium pyruvate MEM (100 m /. Gibco), and 5 mL Penicillin-Streptomycin-Neomycin (PSN) Antibiotic Mixture (Gibco). 

Dulbecco s modified Eagle medium (DMEM, Invitrogen) with and without 10% fetal bovine serum (FBS, Sigma). 

CFS colony-stimulating factor DMEM Dulbecco s modified Eagle medium... 

Growth medium, Dulbecco s Modified Eagles Medium/Hams F12, supplemented with 10% fetal calf serum and glutamine, penicillin, streptomycin, and fungizone. 

Dulbecco s Modified Eagle medium (DMEM) (Mediatech, Inc.). 

Fig. 5. Reactive oxygen species (ROS) detection in rat Schwann cells in vivo. Cells were cultured in Dulbecco s modified Eagle medium supplemented with 10% fetal bovine serum and either 5 vaM (A) or 30 vaM glucose (B). They were then incubated for 45 min in phosphate buffer saline, pH 7.5, containing 10 M of the ROS-sensitive molecular probe 5- (and 6)-chloromethyl-2, 7 -dichlorodihydrofluorescein diacetate (CM-H DCFD A) and viewed by fluorescence microscopy.

Center for Biologies Evaluation and Research Chinese hamster ovary capillary isoelectric focusing cytomegalovims cell specific perfusion rate dry cell weights dehydrofolate reductase Dulbecco s modified eagle medium... 

DlVIAP (6-dimethylammopurine) 272 DMEM (Dulbecco s modified eagle medium) 740 DM RIE-C transfection reagent 1030 DNA (desoxyribonucleic acid) 628... 

DMEM Dulbecco s modified Eagle medium DPPiV dipeptidyl peptidase IV EGF epidermal growth factor EMA epithelial membrane antigen Epith epithelial... 

Caco-2, C5-0, V79 cells (in Dulbecco s Modified Eagle Medium, DMEM), and Chinese hamster ovary (CHO)-Kl cells (in DMEM/F-12) at passage numbers between 30 and 50, and HepG2 cells (in Minimum Essential Medium) at passage numbers between 80 and fOO, are grown as monolayers in 80-cm culture flasks, which are harvested when they reach 80% confluence to maintain exponential growth. 

Fibroblasts were grown using standard conditions in Dulbecco Modified Eagles Medium with fetal calf serum and fused or cocultivated according to Bml et alJ The fused cells were cultured for three days in Dulbecco s Modified Eagle Medium without fetal calf serum after which overall pristanic acid P-oxidation was measured as described... 

Tissue-culture media endotoxin-free Dulbecco s modified Eagles medium (DMEM), minimal essential medium-alpha (MEMa) or Hepes-buffered RPMI-1640 supplemented with 2 mM r-glutamine, and heat-inactivated fetal bovine serum (FBS, to 10% final concentration) (see Note 1). Store at 4 °C. 

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