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Dulbecco’s modified eagle medium DMEM

Appropriate culture medium for the specific tumor cell line(s) under test (e.g., Dulbecco s Modified Eagle Medium (DMEM) lx liquid GlutaMAX I, 4,500 mg/L o-glucose, sodium pyruvate DMEM/F12 (1 1) lx liquid with GlutaMAX I). [Pg.258]

Dulbecco s Modified Eagles Medium (DMEM) containing antibiotics (100 U/mL penicillin, 100 U/mL streptomycin, 0 25 pg/mL amphotericin B, 50 pg/mL genta-mycin) and 10% (v/v) fetal bovine serum (FBS). [Pg.431]

Dulbecco s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and TrypLE Express Stable Trypsin-Like Enzyme with Phenol Red (Invitrogen, Carlsbad, CA). [Pg.237]

Dulbecco s modified Eagle medium (DMEM, Invitrogen) with and without 10% fetal bovine serum (FBS, Sigma). [Pg.103]

Dulbecco s Modified Eagle medium (DMEM) (Mediatech, Inc.). [Pg.325]

Caco-2, C5-0, V79 cells (in Dulbecco s Modified Eagle Medium, DMEM), and Chinese hamster ovary (CHO)-Kl cells (in DMEM/F-12) at passage numbers between 30 and 50, and HepG2 cells (in Minimum Essential Medium) at passage numbers between 80 and fOO, are grown as monolayers in 80-cm culture flasks, which are harvested when they reach 80% confluence to maintain exponential growth. [Pg.515]

Tissue-culture media endotoxin-free Dulbecco s modified Eagles medium (DMEM), minimal essential medium-alpha (MEMa) or Hepes-buffered RPMI-1640 supplemented with 2 mM r-glutamine, and heat-inactivated fetal bovine serum (FBS, to 10% final concentration) (see Note 1). Store at 4 °C. [Pg.148]

Figure 3.7 Osteoblast adhesion on titania of various grain sizes. Rat calvarial osteoblasts (3500 cell/cm ) in Dulbecco s modified Eagle medium (DMEM), containing 10% fetal bovine serum, were allowed to adhere on the surfaces of various grain size titania and borosilicate glass (reference material) under standard cell culture conditions (37 °C, humidified, 5% COj/95% air environment) for4h. Values are mean SEM n=3 p<0.01 (compared to reference glass). Figure 3.7 Osteoblast adhesion on titania of various grain sizes. Rat calvarial osteoblasts (3500 cell/cm ) in Dulbecco s modified Eagle medium (DMEM), containing 10% fetal bovine serum, were allowed to adhere on the surfaces of various grain size titania and borosilicate glass (reference material) under standard cell culture conditions (37 °C, humidified, 5% COj/95% air environment) for4h. Values are mean SEM n=3 p<0.01 (compared to reference glass).
Actively growing 9.5 x 10 CHO-Kl cells were plated in a 100 mm plate using Dulbecco s modified Eagle medium (DMEM) containing 10% fetal bovine serum and proline (0.034 g/liter) and were cultivated for 24 h. [Pg.362]

Tumoral cell lines were tested in the usual manner. The cell lines were trypsinized, cells suspended and counted, then diluted to 10 and plated onto Corning 1 ml well plates. The plates were incubated overnight in a 00% humidity carbon dioxide incubator and the next day the Dulbecco s Modified Eagle Medium (DMEM) sucked off and substituted with DMEM containing various microgram quantities of the platinum-containing polymer. Inhibition was tested for by both visual observation and employing trypan blue (an exclusion stain). [Pg.139]

Swiss 3T3 fibroblast morphology 48 h postseeding on PNIPAM brushes of thickness (a) 3 nm, (b) 5 nm, and (c) 37 nm. Swiss 3T3 cells were seeded at 1(P cells/cm and cultured in Dulbecco s modified eagle medium (DMEM) supplemented with 10% fetal bovine serum, 2% L-glutamine, 1% penicillin (100 U/ml), and 1% streptomycin (100 U/ml) for 48 h. Cells were observed at 37°C using a Leitz Orthoplan microscope in bright field. Scale bar = 100 pm. [Pg.60]

Cell culture medium (Dulbecco s modified eagle medium, DMEM), phosphate buffered saline (PBS), and other substances for cell culture were purchased from Invitrogen Corporation, Grand Island, NY, USA. [Pg.338]

Human embryonic palatal mesenchymal cells (HEPM, ATCC CRL-1486) were seeded onto the coated surfaces in multiple 24-well tissue culture plates (50,000 cells/well). The cultures were maintained in Dulbecco s modified eagles medium (DMEM) supplemented with fetal bovine serum (FBS, 10%), L-glutamine (2 pmol/ml), penicillin G (100 U/ml), streptomycin sulfate (100 pg/ml) and amphotericin B (0.25 pg/ml). Replicate cultures were incubated at 37 C for 6, 24 and 72 hours and then harvested for analysis. [Pg.116]

Dulbecco s Modified Eagles Medium (DMEM) (Sigma D5796)... [Pg.227]

This cell was cultured in a medium composed of Dulbecco s modified Eagle medium (DMEM) 90%, precolostrum newborn calf serum (PNCS) 5%, and horse serum (HS) 5%. The medium for the cell diferentiation was prepared by... [Pg.614]


See other pages where Dulbecco’s modified eagle medium DMEM is mentioned: [Pg.686]    [Pg.166]    [Pg.247]    [Pg.59]    [Pg.215]    [Pg.412]    [Pg.519]    [Pg.736]    [Pg.554]    [Pg.3]    [Pg.11]    [Pg.205]    [Pg.206]    [Pg.81]    [Pg.201]    [Pg.171]    [Pg.205]    [Pg.206]    [Pg.180]    [Pg.22]   
See also in sourсe #XX -- [ Pg.12 , Pg.68 , Pg.69 , Pg.346 , Pg.356 , Pg.357 , Pg.362 , Pg.363 ]




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Dulbecco’s modified Eagle

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