DsDNAs

DNase I (pancreas) DNA a Preferably between Py and Pu nicks dsDNA, creating 3 -OH ends... 

When coRI encounters this sequence in dsDNA, it causes a staggered, double-stranded break by hydrolyzing each chain between the G and A residues ... 

FIGURE 11.33 Restricdon mapping of a DNA molecule as determined by an analysis of the electrophoretic pattern obtained for different restriction endonuclease digests. (Keep in mind that a dsDNA molecule has a unique nucleotide sequence and therefore a definite polarity thus, fragments from one end are distinctly different from fragments derived from the other end.)... 

In principle, the Maxam-Gilbert method can provide the total sequence of a dsDNA molecule just by determining the purine positions on one strand and then the purines on the complementary strand. Complementary base-pairing rules then reveal the pyrimidines along each strand, T complementary to where A is, C complementary to where G occurs. (The analogous approach of locating the pyrimidines on each strand would also provide sufficient information to write the total sequence.)... 

If chromatin is swelled suddenly in water and prepared for viewing in the electron microscope, the nucleosomes are evident as beads on a string, dsDNA being the string (Figure 12.28). The structure of the histone octamer core has been determined by X-ray crystallography without DNA by E. N. Moudrianakis s laboratory (Figure 12.29) and wrapped with DNA by T. J. 

CpG-rich dsDNA, respectively. Following activation, these TLRs bind to a complex consisting of the specific adaptor molecule MyD88, TRAF6, and the kinases IRAK-1 and IRAK-4. ERF-7, which is also part of this complex is then phosphorylated by IRAK-1, forms dimers and shuttles to the nucleus to specifically activate the EFN-a promoter. 

Billich A (2003) S-1360 Shionogi-GlaxoSmithKline. Curr Opin Investig Dmgs 4 206-209 Black LW (1988) DNA packaging in dsDNA bacteriophages. In Calendar R (ed) The bacteriophages. Plenum, NY, pp 321-373... 

Fig. 4.7 Five different types of PNA-dsDNA complexes. DNA is shown schematically as a ladder, and the PNA oligomers are shown in bold...

Triplex forming bis-PNA Globin gene (dsDNA) Electroporation None Monkey kidney CVl mRNA level (RT-PGR) [76]... 

Nielsen P.E., Egholm M., Buchardt O. Evidence for (PNA)2/DNA triplex structure upon binding of PNA to dsDNA by strand displacement. J. Md. Recognit. 1994 7 165-170. 

At the origin of replication (ori), there is an association of sequence-specific dsDNA-binding proteins with... 

Unwinding (denaturation) of dsDNA to provide an ssDNA template. 

Single-strand binding proteins Prevent premature reannealing of dsDNA... 

Two proteins are initially involved in the nonho-mologous rejoining of a ds break. Ku, a heterodimer of 70 kDa and 86 kDa subunits, binds to free DNA ends and has latent ATP-dependent helicase activity. The DNA-bound Ku heterodimer recruits a unique protein kinase, DNA-dependent protein kinase (DNA-PK). DNA-PK has a binding site for DNA free ends and another for dsDNA just inside these ends. It therefore allows for the approximation of the two separated ends. The free end DNA-Ku-DNA-PK complex activates the kinase activity in the latter. DNA-PK reciprocally phos-phorylates Ku and the other DNA-PK molecule, on the opposing strand, in trans. DNA-PK then dissociates from the DNA and Ku, resulting in activation of the Ku helicase. This results in unwinding of the two ends. The unwound, approximated DNA forms base pairs the extra nucleotide tails are removed by an exonucle-... 

Molecular recognition 113 0 t Q Protein, ssDNA, dsDNA, tRNA, rRNA, mRNA, nRNA, bilayers, ligands, cofactors, metals, autoregulatory... 

Fluorescence studies and the binding interaction of Quartz/APES/RB with single- and double-stranded oligonucleotides (ssDNA and dsDNA) in Tris-HCl buffer solution of pH 7.4 were carried out. Quartz/APES/RB exhibited emission at 576 nm, whereas Quartz/APES without BB where nonfluorescent, suggesting that RB successfully assembled on the surface of quartz wafers. By comparison with Aem of 5 x 10 5 M RB solution, which was 588 nm, a hypsochromic shift was found. Considering the fluorescence microscopical image of Quartz/APES/RB, it... 

Overall, Quartz/APES/RB allowed for extremely high sensitive fluorescence recognition for ssDNA and dsDNA with the detection limit of 2.4 ngL 1 and 0.85 ngL-1, respectively. 

Christensen UB, Pedersen EB (2002) Intercalating nucleic acids containing insertions of 1-0-(l-pyrenylmethyl)glycerol stabilisation of dsDNA and discrimination of DNA over RNA. Nucleic Acids Res 30 4918 1925... 

BioMarker products for accurate sizing of dsDNA bands over different ranges, with nine bands of equal ethidium bromide intensity. 

Dissolve the DNA sample to be modified at a concentration of 20-100 pg/ml in 10 mM Tris, 1 mM EDTA, pH 7.4. Note The sample may be heated to denature and solubilize genomic DNA and then cooled to form dsDNA for modification. 

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