Genes dsDNA

Triplex forming bis-PNA Globin gene (dsDNA) Electroporation None Monkey kidney CVl mRNA level (RT-PGR) [76]... 

Vaccinia dsDNA Allows insertion of large genes. Minimum adverse reactions. Anticancer effects of immunogenicity Immunogenic. Limited to non-smallpox vaccinated or immunodeficient subjects... 

The second approach to sequencing RNAs, via their cDNA transcripts, is exemplified by the sequence analysis of the influenza virus haemagglutin gene (Sleigh et al., 1979). In this method a double-stranded (dsDNA) copy of the RNA genome was inserted, after digestion with nuclease Si, into the double-stranded bacterial plasmid pBR322 which was subsequently used to transform a... 

An ESI mass spectrum of a mixture of 1 1 d(pT)13 d(pA)14 with the gene V protein (Fig. 13a) shows the existence of a double-stranded dimer and of a complex between the protein and a single d(pT)13 oligonucleotide. Complexes of the protein with the dsDNA, or with d(pA)14 do not appear in the spectrum. This clearly points to the preference for binding ssDNA over dsDNA and poly(dT) over polyfdA). When the mixture of d(pT)13 d(pT)15 ... 

The transfection of cis-element dsDNA, decoy ODNs, have been reported as powerful tools for gene therapy.Delivered to cells, decoy ODNs containing a... 

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