Drugs immunosuppresive

Mueller XM. Drug immunosuppresive therapy for adult heart transplantation. Part I Immune response to allograft and mechanism of action of immimosuppressants. Ann Thorac Surg 2004 77 354-362. 

Inhibitors. Aside from its role in providing reduced folate coenzymes for cells, this enzyme has attracted a great deal of attention because it appears to be a site of action of the important anticancer drugs methotrexate (amethopterin) and aminopterin.293 364 365 These compounds inhibit dihydrofolate reductase in concentrations as low as 10 8 to 10 9 M. Methotrexate is also widely used as an immunosuppresant drug and in the treatment of parasitic infections. 

In situations where the analyte is poorly soluble in aqueous buffers, the use of organic solvents may even be beneficial. One such example is determination of cyclosporin, which is a potent immunosuppresive drug used in transplant surgery... 

Inhibition of oxazolone-induced delayed-type hypersensitivity is used as an in vivo test for inhibition of the cellular immune response. The shaved abdominal surfaces of groups of mice are sensitized by topical application of 0.1ml of 5% oxazolone. The test drug is given intraperitoneally an hour later and then daily for five consecutive doses. After an additional 4 days, the animals are challenged by application of 0.5 ml oxazolone to one ear. Ear thickness is measured 24 h later. A decrease in ear thickness relative to untreated controls is an indication of immunosuppresion. 

Gluckman E, Devergie A, Lokiec F, de Castro H, Meletis J, Traineau R, Cosset JM, Le Mercier N, Varrin F, Cavazzano M. Role of immunosuppresive drugs for prevention of graft-host disease after human HLA matched bone marrow transplantation. Transplant Proc 1987 19 S61-S65. 

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