Downstream processing resolution

High-resolution chromatography normally yields a protein that is 98-99 per cent pure. The next phase of downstream processing entails formulation into final product format. This generally involves ... 

Product manufacture entails viral vector propagation in a suitable animal packing cell line (known as HEK 293). After cell recovery and lysis, the crude product is clarified by filtration and concentrated by ultrafiltration. The product is then treated with a nuclease preparation in order to degrade contaminant DNA and further downstream processing entails multi-step high-resolution column chromatography (see also Figure 14.7). 

The IL/SC-CO2 media for 1-phenylethanol resolution, catalyzed by CALB, offers a number of advantages, such as high resolution yields, simple downstream processing, guarantying an enzyme overstabilization effect and exceptional enzyme enantioselectivity with no transformation of the not desired enantiomer, albeit apparently lower conversions were registered in this medium compared to the ones, obtained in SC-CO2. 

The characterization of multistage assembly line action by high-resolution MS was developed in particular on yersiniabactin assembly line components. Five of its active sites were detected in parallel to monitor intermediate downstream processing on a biosynthetic thiotemplate.4... 

Downstream processing is commonly classified into four distinct steps broth conditioning and removal of insolubles isolation of the desired product (including clarification and extraction) purification with high-resolution techniques and polishing. 

---