Downstream Fuses

If the transformer is HV/HV, the same requirement must prevail, i.e. fora fault on the dowmstream (secondary side) only the downstream fuses must operate and not the fuses on the itpstream (primary side). 

Very and extremely inverse relays are used in systems where the fault level downstream is low when compared with that available at the point of main supply, e.g. a main generator switchboard. They are also nsed where coordination with upstream or downstream fuses is necessary. 

The SH2 DOMAIN links an activated (autophosphorylated) receptor to various downstream pathways. Each of the proteins has a common SH2 adaptor domain fused to another domain that performs the downstream function. The same signal can then activate multiple pathways. GNRP and GAP represent adaptors that can affect G-protein signaling (see the following section G-Protein Coupled Receptors ). 

The drilling operation, which provides a recess for the installation of a fuse in a 155mm shell, was performed in building 1619. An air driven drill is used to put a recess in the Composition B that has been encased in the nose. The dust generated from this operation is removed by suction through a 5.1 cm (2.0 in) line to a Hoffman primary dust collector. Downstream of the primary collector is a secondary collector used to take any excess not trapped in the primary collector. Two sample areas were selected for study as shown in Figure 5. 

The fusion method is very advantageous for downstream processing, especially when the fused protein is produced in soluble form, thus circumventing the need for renaturation. In addition, it allows the insertion of affinity tails or tags by fusion of... 

In general, the MC2 receptor is Gs protein-coupled (Table 1), and two studies in the rabbit pulmonary artery indicate that this is also true for the presynaptic receptor. The evidence is, indirect, however, in that it suggests activation of adenylyl cyclase, the typical transduction step downstream from Gs. In the study by Gothert and Hentrich (1984), the facilitatory effect of ACTH was increased by simultaneous administration of forskolin, an activator of the catalytic subunit of adenylyl cyclase, and AH 21-132, a phosphodiesterase inhibitor. In the study by Costa and Majewski (1988), the facilitatory effect of ACTH was occluded when the vessel was super-fused with a lipid-soluble cAMP analogue. 

Nrg1tm2Cbm Targeted mutation 2, Carmen Birchmeier Exon 8 is fused to a lacZ gene. Coding sequences downstream of the fusion point, including the N-terminal half of the EGF domain, are deleted... 

Fig. 4.1-6 The bacterial two-hybrid system and Y. Binding of the Acl repressor to the X developed by Hochschild and coworkers. operon followed by dimerization of X and Y The Acl repressor and the a-subunit of recruits RNAP leading to transcription RNAP are fused to two arbitrary proteins, X activation of a downstream reporter gene.

A very common and powerful application is creating an inducible allele of a protein in order to dissect its function. Typically, the protein of interest is fused to a dimerization domain, cells expressing the fusion protein are exposed to dimerizer, and the consequences are assessed by any appropriate technique, such as assaying downstream signaling or profiling mRNA expression. The key advantages of chemically induced dimerization are that activation can be restricted to one particular protein and can be initiated and then monitored in real time by addition of drug. This allows very specific questions to be asked about the function of a protein or of the pathway that it controls. 

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