Dopaminergic cell line

Tang L, Todd RD, Heller A, O Malley KL (1994b) Pharmacological and functional characterization of D2, D3 and D4 dopamine receptors in fibroblast and dopaminergic cell lines. J Pharmacol Exp Ther 268 495-502. 

Both isoforms induce a Pi-linked mobilization of intracellular calcium, when expressed in fibroblast cells, identical in response and pharmacology, and sensitive to pertussis toxin (PTX) treatment, indicating that a coupling of Gaj/Ga0 proteins were responsible (Vallar et al., 1990). Activation of protein kinase C blocked D2S-mediated increase in calcium, whereas D2L response was considerably more resistant (Liu et al., 1992a). However, activation of D2R in a dopaminergic cell line did not affect PI hydrolysis (Tang et al., 1994) or rat striatal slices (Kelly et al., 1988 Rubinstein and Hitzemann, 1990), but actually inhibited hydrolysis in pituitary cells (Simmonds and Strange, 1985 Enjalbert et al., 1990). 

Cavanaugh, J.E., Jaumotte, J.D., Lakoski, J.M. Zigmond, M.J. (2006). Neuroprotective role of ERKl/2 and ERK5 in a dopaminergic cell line imder basal conditions and in response to oxidative stress. Journal of Neuroscience Research, 84, 1367-1375. 

Batchelor PE, Liberatore GT, Wong JY, Porritt MJ, Frerichs F, Donnan GA, Howells DW (1999) Activated macrophages and microglia induce dopaminergic sprouting in the injured striatum and express brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. J Neurosci 79 1708-1716. 

Rosenblad C, Martinez-Serrano A, Bjorklund A (1998) Intrastriatal glial cell line-derived neurotrophic factor promotes sprouting of spared nigrostriatal dopaminergic afferents and induces recovery of function in a rat model of Parkinson s disease. Neuroscience 62 129 137. 

L. F. Lin, D. H. Doherty, J. D. Lile, S. Bektesh, and F. Collins. GDNF a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons [see comments]. Science, 260 (5), 1130-1132,... 

Mehta V, Hong M, Spears J, Mendez I (1998) Enhancement of graft suiwival and sensorimotor behavioral recovery in rats undergoing transplantation with dopaminergic cells exposed to glial cell line-derived neurotrophic factor. J Neurosurg 88 1088—1095. 

Batchelor PE, Liberatore GT, Ponitt MJ, Dorman GA, Howells DW (2000) Inhibidon of brain-derived neurodophic factor and glial cell line-derived neurodophic factor expression reduces dopaminergic sproudng in the injured sdiatum. Eur J Neurosci 12 3462—3468. 

In vitro systems have been developed to try and understand the mechanism of action of maneb. In particular, the mechanism of toxicity of maneb on the central nervous system using synaptosomal and mitochondrial preparations from brain tissue has been utilized. These studies have shown that maneb has adverse effects on the dopaminergic system, via mechanisms that relate to mitochondrial inhibition and altered neurotransmitter uptake. The genotoxic, cytotoxic, and neurotoxic effects of maneb have been studied using a variety of primary cultures as well as cell lines, including human lymphocytes. As noted above, maneb has little mutagenic potential. 

Bowenkamp, K. E., Hoffman, A. F., Gerhardt, G. A., Henry, M. A., Biddle, P. T., Hoffer, B. J.,andGranholm,A. C., Glial cell line-derived neurotrophic factor supports survival of injured midbrain dopaminergic neurons, J. Comp. Neurol., 355,479,1995. 

Hou, J.G.G. Lin, L-F.H. Mytilineou, C. Glial cell line derived neurotrophic factor exerts neurotrophic effects on dopaminergic neurons in vitro and promotes their survival and regrowth by 1-methyl 4-pyridinium. J. Neurochem. 1996, 66, 74-82. 

Tatard VM, Sindji L, Branton JG, et al. Pharmacologically active microcarriers releasing glial cell line—derived neurotrophic factor survival and differentiation of embryonic dopaminergic neurons after grafting in hemiparkinsonian rats. Biomaterials 2007 28 1978-1988. 

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