DMPC

Fig. IV-19. Fluorescence micrographs showing the shape transitions in monolayers of dimyristoylphosphatidylcholine (DMPC) (84%) and dihydrocholesterol (15%) and a lipid containing the dye, Texas Red. (From Ref. 228.)...

A study by Bames and co-workers of the equilibrium spreading behavior of dimyristol phosphatidylcholine (DMPC) reconciles the differences between spreading of bulk solids and dispersions of liposomes [41]. This study shows the formation of multibilayers below the monolayer at the air-water interface. An incipient phase separation, undetectable by microscopy, in DMPC-cholesterol... 

While most vesicles are formed from double-tail amphiphiles such as lipids, they can also be made from some single chain fatty acids [73], surfactant-cosurfactant mixtures [71], and bola (two-headed) amphiphiles [74]. In addition to the more common spherical shells, tubular vesicles have been observed in DMPC-alcohol mixtures [70]. Polymerizable lipids allow photo- or chemical polymerization that can sometimes stabilize the vesicle [65] however, the structural change in the bilayer on polymerization can cause giant vesicles to bud into smaller shells [76]. Multivesicular liposomes are collections of hundreds of bilayer enclosed water-filled compartments that are suitable for localized drug delivery [77]. The structures of these water-in-water vesicles resemble those of foams (see Section XIV-7) with the polyhedral structure persisting down to molecular dimensions as shown in Fig. XV-11. 

Figure Bl.20.11. Force curves of DMPC/DPPE (dimyristoyl phosphatidylcholine and dipalmitoyl phosphatidylethanolainine) bilayers across a solution of PEG at different concentrations. Clearly visible is a concentration-dependent depletion attraction, with pennission from [17],...

The presence of impurities like free fatty acids in egg or soybean phosphatidylcholine, or in the (semi)synthetic phosphatidylcholines (e.g., DMPC, DPPC, DSPC) can be detected by monitoring the electrophoretic behavior of liposome dispersions of these phospholipids in aqueous media with low ionic strength a negative charge will be found on these liposomes when free fatty acids are present in the bilayers. 

Supported lipid bilayers on planar silicon substrates have been formed using S-layer protein from B. coagulans E38/vl and from B. sphaericus CCM 2177 as support onto which l,2-dimyristoyl-OT-glycero-3-phosphocholine (DMPC) (pure or mixtures with 30% cholesterol) or DPPC bilayers were deposited by the Langmuir-Blodgett-technique (Pig. 

Figure 13.9 (a) Temperature dependence of p on the DMPC bilayer on glass (white) and silicon (black), (b) Dependence ofp on the molar fraction of a cationic DMTAP additive in the DMPC bilayer. Adapted from Ref [48] with permission. 

FIG. 10 Vibrational sum frequency spectrum of saturated monolayers of dilauroyl- (DLPQ, dimyristoyl- (DMPC), dipalmitoyl- (DPPC), and distearoyl-phosphatidylcholine (DSPC) at the D2O-CCI4 interface at ambient temperature in the region of the methylene and methyl symmetrical stretches. (From Ref 139, copyright American Chemical Society.)... 

FIG. 6 Enhancement factor observed in the forward rate constant for TEA+ ion transfer at the water-l,2-DCE interface due to the presence of PCs DLPC ( ), DMPC ( , DPPC (A), and DSPC ( ). (Experimental data are taken from Ref 12.)... 

Temperature 37°C, 0.02 M ionic strength, ultrafiltration method, small unilamellar vesicles (DMPC) [441],... 

Xiang, T.-X. Anderson, B. D., Phospholipid surface density determines the partitioning and permeability of acetic acid in DMPC cholesterol bilayers, J. Membrane Biol. 148, 157-167 (1995). 

FIGURE 2.1 Energy of the 0-0 vibrational transition in the principal electronic absorption spectrum of violaxanthin (l Ag-—>1 BU+), recorded in different organic solvents, versus the polarizability term, dependent on the refraction index of the solvent (n). The dashed line corresponds to the position of the absorption band for violaxanthin embedded into the liposomes formed with DMPC (Gruszecki and Sielewiesiuk, 1990) and the arrow corresponds to the polarizability term of the hydrophobic core of the membrane (n = 1.44). 

DGDG digalactosyl diacylglycerol DHPC dihexadecyl phosphatidylcholine DMPC dimyristoyl phosphatidylcholine DOPC dioleoyl phosphatidylcholine DPPC dipalmitoyl phosphatidylcholine EYPC egg yolk phosphatidylcholine POPC 1-palmitoyl 2-oleoyl-phosphatidylcholine SBPC soya bean phosphatidylcholine... 

FIGURE 10.3 Profiles of the order parameter (order parameter is plotted in a log scale as a function of nitrox-ide position (n) along the alkyl chain of n-SASL) at 25°C in DMPC membranes with and without 10mol% zeaxanthin. (From Subczynski, W.K. et al., Biochim. Biophys. Acta, 1068, 68, 1991. With permission.)... 

FIGURE 10.7 (a) Representative saturation-recovery signals of 14-PC in DMPC membranes containing... 

FIGURE 10.10 (a) Continuous-wave saturation data for the central line of 12-SASL in DMPC membranes at... 

C. Membranes in the absence (O, V) and presence ( , T) of 10mol% lutein, and in the absence (O, ) and presence (V, T) of 50mM K3Fe(CN)6 in the buffer, (b) Relative accessibility parameter obtained at 30°C in DMPC with and without 10 mol% lutein plotted as a function of the position of the nitroxide moiety of SASL in the membrane. (From Wisniewska, A. et al., Biochim. Biophys. Acta, 1368, 235, 1998. With permission.)... 

LUT 14NC5 15NC16 CHOL DMPC 4NC7 15NC16 DMPC POPC... 

LUT DMPC 14NC10 1sNC18 DMPC CHOL, 4NC16 15NC18 POPC... 

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