Dissociating agent

Another injectable anesthetic widely used in feline and primate practice is ketamine hydrochloride [1867-66-9]. Ketamine, a derivative of phencychdine, can be chemically classified as a cyclohexamine and pharmacologically as a dissociative agent. Analgesia is produced along with a state that resembles anesthesia but in humans has been associated with hallucinations and confusion. For these reasons, ketamine is often combined with a tranquilizer. The product is safe when used in accordance with label directions, but the recovery period may be as long as 12—24 h. 

CCP remains attached to the casein following treatment with protein dissociating agents (e.g. urea) or following proteolysis. 

Isolation. Numerous methods have been developed for the purification of XO from milk since the enzyme is concentrated in the MFGM, in which it is one of the principal proteins, all methods employ cream as starting material, use a dissociating agent to liberate XO from membrane lipoproteins and some form of chromatography for further purification. 

The effects of dissociating agents have been described. In 6 M guanidine hydrochloride a value of 83,300 was found (14), but this value was probably the result of partial reassociation (43). Urease dissociates even in 3 M guanidine hydrochloride to (2n) subunits of 60,000 daltons (7), but it reaggregates upon standing. 

The separation by ion exchange can also be done using cationic sorbents. In this case and in the presence of mild dissociating agents (dilute buffered solutions of ethylene glycol and or urea), antibodies will be found in the flowthrough and impurity traces as well as traces of protein A will be adsorbed by ion exchange. 

Halpern JH. Hallucinogens and dissociative agents naturally growing in the United States. Pharmacol Ther... 

The second method of carboxylation also used KMnO as an oxidation agent but, in this case, 18-crown-6-ether was employed as the phase transfer agent and KMnO was employed as the dissociation agent. A reasonable retention of the molecular weight was observed when the pH of the reaction was carefully controlled (Bear et al. 2001 Kurth et al. 2002)... 

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