Disaggregation procedure

Costa, A., Silvestrini, R., Del Bino, G., and Motta, R. (1987) Implications of disaggregation procedures on biological representation of human solid tumors. Cell Tissue Kinet. 20,171-180. 

Schweitzer, P. J., Puterman, M. L., tmd Kindle, K. W. (1985), Iterative Aggregation-Disaggregation Procedures for Discounted Semi-Markov Reward Processes, Operations Research, Vol. 33, pp. 589-605. 

If particles are aggregated after the opsonization procedure, vortex the particles vigorously. If vortexing does not disaggregate the particles, then place the tube containing the particles in an ultrasonic cleaning bath for 10 min. 

Errors in the opposite sense will occur if there is an induction period, e.g., from the presence of an inhibitor which is removed by reacting with the catalyst, from slow initiation or disaggregation of a crystalline solid, and low results will be obtained [20]. A procedure to meet this problem is to extrapolate the yield of polymer [P] against active site concentration since, if polymerization rate and monomer concentration remain constant. 

However, there are more complex situations that need further analysis. Specifically, the case of pathways that are being disaggregated or abstracted and that themselves are not simple pathways from a structural point of view, but are also part-of other pathways in the parent context. What happens with these semantic links in the new context A typical situation is depicted in Fig. 15b, where pathway P, which is part-of pathway P in CONTEXT-i, is disaggregated in pathways P,, Pj g and Pi c in CONTEXT-]. The structural-compatibility procedure, apart from establishing is-disaggregated-in links across the contexts, should also establish is-part-of links between pathways P,, P, g and P, c, and pathway P. 

More formally, the structural-compatibility procedure should perform the following checking operation every time a pathway is disaggregated in a new context. 

A conventional flow cytometry procedure for creating single cell suspensions is provided. Methods of tissue disaggregation to create single-cell suspensions from formalin fixed tissue are also given. Chemical, mechanical, and enzymatic multistep methods for separating single cells from tissue are discussed. These procedures are very tissue dependent. 

Fig. 8.6. Results of an iterative curve separation procedure of a bimodal system of CO/AO-PEC in the stage of salt-caused disaggregation (curve 5 in Fig. 8.5). Master scattering curves of Debye plot (7) experimental points, (2) theoretical scattering curve of the component of large particles, (2) theoretical scattering curve of the disaggregated particles, 4) sum of the scattering intensities corresponding to curve 2 and 3...

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