Dionex

Dionex Corporation - Manufacturers of liquid chromatography systems (IC and HPLC), chromatography software data systems, reversed-phase and ion-exchange columns, and accelerated solvent extraction systems... http //www.dionex.com. 

Ion HPLC Sample dissolved in water, separation on Dionex 480 nm Drinks, instant 0 ... 

The mild-alkali-extract was digested to completion with excess EPG at room temperature for at least 48 hours. Figure 1 shows the separation of the products on a Dionex PAl ion-exchange column. The limit digest of pectic acid (nonesterified HG) by this enzyme is mono-. 

Figure 3. Dionex HPAEC profiles following 45 minute digestion of 1.25% PGA by 0.1 RGU each of 3 different glycoforms of F. Moniliforme EPGs.

Figure 4. Dionex HPAEC profiles following 45 minute digestion of 1.25% PGA by 0.1 RGU of F. moniliforme EPG expressed in Saccharomyces cerevicae (YPG) or the deglycosylated form (dYPG). This convinced us that, at least for Fusarium EPG, the glycosylation played no role in changing its ability to hydrolyze PGA.

Figure 6. Dionex HPAEC profiles following 48 hour digestion of 1.25% PGA by 0.1 RGU each of 4 different EPGs inhibited by cv cannelino cultured cell PGIP. A and B) F. moniliforme EPG glycoforms, C) deglycosylated S. cerevicae expiressed EPG, D) S. cerevicae expressed EPG.

HPAEC analyses were carried out to determine the oligomeric products released from various pectic substrates after depolymerization by the PL isoenzymes. Action pattern analyses for the concerted action of PL isoenzymes utilized 68% esterified pectin as substrate. One-ml reaction mixtures in a buffer system as detailed in section 2.2. comprising 0.5% (w/v) substrate and 5 U of enzyme were incubated for 30 s to 18 h, and then thermoinactivated. Samples of 750 pi were applied to a Carbopac PA-1 (Dionex) column before the carbohydrates were eluted over a period of 70 min using a gradient of 0.2 M KOH, 0.05 M K-acetate to 0.2 M KOH, 0.7 M K-acetate. Detection employed a Pulsed Electrochemical Detector (PED, Dionex) in the integrated amperometry mode according to the manufacturer s recommendations. 

Figure 1. Time-course measurement of polygalacturonase (PG) activity in the culture filtrate of Colletotrichum lindemuthianum grown on pectin and HPLC-Dionex analysis of mono-, di- and tri-galacturonic acid residues simultaneously released in the culture medium. The data are the mean of three independent experiments or represent one typical experiment in the case of galacturonic acid (GalA) residues. DP= Galacturonic acid degree of polymerization.

Stachyose and raffinose were analysed according the the recommedation of Dionex... 

Breakdown products characterization fay HPLC-Dionex chromati rai. ... 

The Dionex system uses a Garbo Pac PA-1 anion exchange column and a CarboPac PA-1 Guard. The column was loaded with 25 pi of the RG solution and eluted with a linear gradient of 0 - 0.5 M NaOAc in 0.1 N NaOH during 50 minutes. The flow rate was 1.0 ml/min and the process was monitored using a PE detector. 

Dionex analysis of hydrolysis products of RG by these recombinant strains (see figure 4 ) shows the formation of various oligomers as published by Schols d al.(1992). 

From these results it was concluded that the DNS method can be used as a method to optimize and follow the production-process of RGase by the wild strain. Dionex analysis is a technique which provides us to control the production of the ri t enzyme but does not give any indication about activity. 

Figure 4 Dionex patterns of RG treated with different strains.

Sugar analysis was performed after methanolysis combined with TFA hydrolysis using a Dionex Bio-LC system (Sunny vale, CA) equipped with a CarboPac PAl column (4 x 250 mm). The experimental conditions were the same as those used by Ruiter et. al. [18]. 

Oligosaccharides were isolated preparatively by high-pH anion exchange chromatography carried out on a LC-system (Dionex Corporation, Sunnyvale CA) equipped with a CarboPac PA-1 column (9 x 250 mm), coupled to a Spectra System AS 3500 auto sampler. The detection was carried out using Pulsed Amperometric Detection (PAD-II). 

Oligalacturonides were separated on a Dionex Bio LC with a CarboPac PA 1 column and pulsed amperometric detection using a linear Na-acetate-gradient in 0.1 M NaOH. 

Pood Technology Center Danisco Ingredients Dionex B. V. 

Garimella et investigated the effect on trifluralin recovery of different extraction methods. A supercritical fluid extraction (SEE) procedure for the isolation of the analytes from the matrices with a commercial SEE system (Dionex Model 703)... 

Dionex Accelerated Solvent Extractor (ASE200) with 11- and 22-mL extractor cells... 

---