Dinuclear Metallohydrolases

Daumann, Spectroscopic and Mechanistic Studies cf Dinuclear Metallohydrolases and Their Biomimetic Complexes, Springer Theses, DOI 10.1007/978-3-319-06629-5 l, Springer International Publishing Switzerland 2014 

Enzyme Metal ion composition Biological function Research incentive 

Glycerophospho-diesterase (GpdQ) [2-10] Fe(II)-Zn(II), Co(II)-Co(II), Mn(II)-Mn(II), Cd(II)-Cdai) Hydrolysis of 3 -5 phosphodiester bond of glycerophosphodiesters Potential bioremediator 

Purple acid phosphatases (PAP) [11-19] Fe(III)-Fe(II), Fe(III)-Mn(II), Fe(III)-Zn(II) Bone metabolism (animals) and phosphate uptake (plants) Target for anti-osteoporotic drugs 

Metallo-P-lactamases [20] Zn(II)-Zn(II) Hydrolysis of P-lactam substrates (antibiotics) Study potential inhibitors to fight antibiotic resistance 

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Figures and Tables in this chapter have appeared in [L.J. Daumann et al., Spectroscopic and mechanistic studies of dinuclear metallohydrolases and their biomimetic complexes Dalton Trans. 2014, 43, 910-928.]—Reproduced by permission of The Royal Society of Chemistry.

In this work a detailed study of the enzyme GpdQ and biomimetics of dinuclear metallohydrolases will be conducted. The specific aims of this thesis are ... 

In order to generate more structurally relevant biomimetics for dinuclear metallohydrolases much effort has been devoted to the synthesis of asymmetric ligands. These ligands are considered to be more suitable models for the asymmetric coordination environment found in enzymatic systems. Nordlander et al. proposed that asymmetric complexes are not only more appropriate functional models for the active site of phosphoesterase enzymes, but also that they exhibit enhanced catalytic rates compared with their symmetric counterparts [1-3]. A selection of ligands used to generate purple acid phosphatase [1, 4, 5, 6-10], phosphoesterase [11], urease [12, 13], catechol oxidase [14] and manganese catalase biomimetics [15, 16] is displayed in Fig. 7.1. 

L. J. Daumann, Spectroscopic and Mechanistic Studies of Dinuclear Metallohydrolases 189... 

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