2.4- Dinitrobenzene sulfonate

Sodium hydroxyalkanesulfonates may be determined in the presence of an unsaturated hydrocarbon, including sodium alkenesulfonate. The sulfonates are converted to the free sulfonic acids using a slight excess of 2,4-dinitrobenzene-sulfonic acid. The hydroxyl group of the sulfonic acid liberated is acetylated in ethyl acetate solution by a known excess of acetic anhydride. The unconsumed anhydride is hydrolyzed by a pyridine-water mixture and the acids titrated potentiometrically with standard sodium hydroxide solution. The hydroxy-alkanesulfonate content is calculated after correction for any traces of acidity or alkalinity in the original sample. 

Benzoselenadiazole (1) is alkylated by dialkyl sulfates and dialkyl 2,4-dinitrobenzene sulfonates to give 1-alkyl 2,1,3-benzoselenadiazolium salts <89SC1381>. 2,1,3-Benzoselenadiazole (1) reacts with I2, KI and HCl in CHCI3 to give the quasi-polymeric structure (9) <85ZAAC(523)33> (see Section 4.21.3.1). 

Wistar-derived male or female rats weighing 200 g, which fasted 24 hours, were used. Distal colitis was induced by intracolonic instillation of 2,4-dinitrobenzene sulfonic acid, which was injected to ensure that the solution remained in the colon. The Step 3... 

Fig. 12.1. Some active molecules used for the haptenation of antibodies. In (a) /i-aminobenzenearsonic acid or A -(4-aminobenzoyl)-L-glutamic acid are diazotized (according to Table 12.4) and reacted separately with 4-hydroxyphenylisothiocyanate to yield ars-azo-hydroxyphenylisothiocyanate (RAPITC) and glut-azo-hydroxyphenyl-isothiocyanate (GAPITC), respectively, which differ in R. 2,4-Dinitro-l-fluorobenzene and 2,4-dinitrobenzene sulfonate are presented in (b).

Edwards and colleagues have reported derivatization with 2,4-dinitrobenzene sulfonic acid, a procedure useful for quantification of a number of phenylethylamines, to be unsuitable for analysis of HA. However, Doshi and Edwards discovered that 2,6-dinitro-4-trifluoromethyl benzenesulfonic acid (DNTS) reacts readily with catecholamines, histamines, and related biogenic amines with very suitable properties for GC-ED. Formation of the trifluoroacetyl, trimethylsilyl, and heptafluoro butyryl derivatives of HA for GC have been reported, but these compounds have been found to be unsuitable for quantification of HA because of excess tailoring. 

A simple test for free epoxy groups, either in epoxy monomers or in only partially reacted resins, is the reaction with 2.4-dinitrobenzene sulfonic acid. In the presence of piperazine, the resulting ester gives an intensive yellow coloration. For this test, about 0.5 g of the sample is dissolved in a few ml of dioxane, and then a 1 % solution of 2.4-dinitrobenzene sulfonic acid in dioxane is added. After about 1 hr, add a 1 % solution of piperazine in dioxane. The yellow color is specific for free epoxy groups. 

Epoxy groups have also been determined spectrophotometrically using 2,4 dinitrobenzene sulfonic acid [66] and by the Zeisel method [67-69]. 

Potassium 4-chloro-3,5-dinitrobenzene-sulfonate, 31, 46 Potassium cyanate, 31, 9 Potassium cyanide, 30, 84 32, 31, 63 Potassium ethyl xanthate, 30, 56 Potassium hydroxide, 30, 103 Potassium iodide, 30, 34 31, 31, 66 Potassium methyl sulfate, 31, 73... 

Potassium 4-chloro-3,5-dinitrobenzene-sulfonate, 31, 46 Potassium cyanate, 31, 9 Potassium cyanide, 30,84 32,31,63 37,47 Potassium ethyl malonate, 37, 34 Potassium ethyl xanthate, 30, 56 Potassium fluoride, 36, 40 Potassium iodide, 30, 34 31, 31, 66 Potassium metal, 37, 29, 30 Potassium methyl sulfate, 31, 73 Potassium nitrate, 31, 46 Potassium 1-nitropropylnitronate, 37, 24 Potassium oxalate, 34, 83 Potassium permanganate, 30, 87 31, 59 Potassium sulfide, 32, 103 Potassium thiobenzoate, 32, 101 Potassium thiocyanate, 32, 39, 40 Prins reaction, 33, 72 Propane, 1, 3-dibromo-2, 2-Ws-(bromo-methyl)-, 31, 82... 

Kurz, L.C. and Erieden, C. (1977). Comparison of the structures of enzymatic and nonenz3fmatic transition states. Reductive desulfonation of 4-X-2,6-dinitrobenzene-sulfonates by reduced nicotinamide adenine dinucleotide. Biochemistry 16, 5207 -5216... 

I. Treatment of Crohn s Disease Using Dinitrobenzene Sulfonic Acid-induced Inflammatory Bowel Syndrome... 

Potassium amide, 39, 73 Potassium 4-amino-3,5-dinitrobenzene-sulfonate, 31, 46... 

Dinitrobenzene-sulfonate (DNBS) is recrystallized from ethanol by dissolving its sodium or potassium salt (15 g) in 950 ml ethanol at 70 C with vigorous stirring. Norit A (2 g) is added, the mixture is stirred and then filtered rapidly through several layers of filter paper (Whatman No. 3) on a preheated funnel. Cooling causes the formation of small crystals. This procedure is repeated 2-4 times. The crystals (mp>300 C) are dried at 37 C and stored at 4 C in a dark container. 

T. spiralis Infection ameliorates experimental (dinitrobenzene sulfonic acid = DNBS)-induced colitis in mice, with a down-regulation of myeloperoxidase (MPO) activity in colonic tissue linked to an emerging Th2-type immune response characterized by high lL-4 and lL-13 production by spleen cells in T. spiralis infected mice (Khan et al. 2002). 

Phosphorus pentachloride, 31, 48, 104 Phosphorus pentoxide, 30, 22, 46 Phosphorus trichloride, 31, 88, 111 Potassium, 30, 19, 20 directions for safe handling of, 30, 20 Potassium 4-amino-3,5-dinitrobenzene-sulfonate, 31, 46... 

Oryzalin is prepared by a different route. Synthesis starts with chlorobenzene, which is nitrated in a single step into potassium 4-chIoro-3,5-dinitrobenzene sulfonate (I). This is then converted with phosphorus oxychloride and phosphorus pentachloride into 4-chloro-3,5-dinitrobenzene sulfonyl chloride (II). The reaction of the latter with dipropylamine gives 4-dipropylamino-3,5-dinitrophenyl sulfonyl chloride (III), the reaction of which with ammonium hydroxide yields oryzalin. 

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