Diglyceride preparation

The plasmalogenic diglyceride, prepared enzymically by the action of phospholipase C on the choline-containing phospholipid of beef heart, is defined as a D-a,/5-diglyceride in which the ester at the cx -position is replaced by an imsaturated ether. The reaction is catalysed by a choline-phosphotransferase enzyme similar to the phosphorylcholine-glyceride transferase shown to participate in the biosynthesis of lecithin (Reaction 12). A similar reaction occurs in brain (McMurray 1964b). 

Temperature, on the other hand, has no influence on the ratio of reaction products but influences the rate of reaction and the forming of colored byproducts [14]. The preparation of surfactants by reaction of P4O10 with fats, oils, and diglycerides is described in early patents [1,15,16]. Surfactants were prepared by the neutralization of higher fatty alcohol-mixed phosphates [17]. 

Figure 8 shows a comparison of the HPLC chromatograms of rape-seed oil between one- and two-step preparation methods. As a comparison, the HPLC chromatogram of rapeseed oil treated with supercritical methanol in the presence of water is also shown. It is obvious that peaks of the two-step method only consist of methyl esters, whereas for other supercritical methanol methods, some peaks of intermediate compounds such as monoglycerides and diglycerides as well as unreacted triglycer-... 

Glycerolysis has been used to prepare mono- and diglyceride emulsifiers by reacting TAG with an excess of glycerol.44... 

Preparing an oil-soluble additives mixture containing mono- or diglycerides and other emulsifiers, flavorings, oil-soluble vitamins, and yellow color at the margarine/spread plant. 

Calculation Calculate the percent of diglycerides in the Sample Preparation by the following formula ... 

Fig. 59.—Anti-firming effect of the monoglyceride preparations in white bread. Diglyceryl octadecanoate (DGMS) at pH 6.8 (open points), at pH 7.3 (solid points) DGMS hydrate (solid squares) DGMS hydrate powder (open squares) DGMS powder (solid triangles) mono- and diglyceride emulsion (open triangles) control (crosses). (Reprinted with permission from N. Krog and B. N. Jensen, J. Food Technol., 5 (1970) 77-87.)...

Newton JM. Pinto MR. Podczeck F. The preparation of pellets containing a surfactant or a mixture of mono and diglycerides by extrusion/spheronization. Eur J Pharm Sci 2007 66 83-94. 

The conclusion that PS is essential for enzyme activity has been challenged by De Pont et al. [116]. They have quantitatively removed PS and PI without delipidating the enzyme preparation from rabbit kidney outer medulla. A purified enzyme preparation was treated with PS-decarboxylase converting all PS to phosphatidyl eth amine or with Pl-specific phospholipase C converting all PI to the diglyceride... 

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