P-Chloromercuriphenyl sulfonate

Table 7. Oxidation state of the iron in oxidized bacterial and plant ferredoxin in the presence and absence of p-chloromercuriphenyl sulfonic acid (PCMS)...

NMR, nuclear magnetic resonance mV, millivolt pCMB, p hloromercuribenzoate pCMS, p-chloromercuriphenyl sulfonate PMS, phenazine methosulfate EDTA, ethylenediaminetetraacetate SDS, sodium dodecyl sulfate TTFA, 2-thenoyltriflu-oroacetone ETP, submitochondrial (electron transfer) particles MVH, reduced methyl viologen DEAE-cellulose, diethylaminoethyl cellulose succ, succinate APS, adenosine S -phosphosulfate PAPS, 3 -phosphoadenosine 5 -phosphosulfate. Other abbreviations are standard [see JBC 244, 2 (1969)]. 

Chloroethanol, catalase and, 401 p-Chloromercuriphenyl sulfonate glutathione reductase and, 141 small NADH dehydrogenase and, 203 thioredoxin reductase and, 146 ubiquinone reductase and, 197 Chloroplasts, transhydrogenase of, 66 Chlorosuccinate, succinate dehydrogenase and, 237-238 Cholate... 

Glutathione reductase from yeast and from E. coli contains 4 and 5 thiol groups per FAD, respectively (Table I), in addition to the active center disulfide. Peptides containing 3 of the 4 thiols have been isolated and sequenced Cys-AsnAsp Lys-Ile-Ala-Cys-Pro-Gly-Asn-Val-Glo-Lys Asp-Thr-Ile-Tyr- (His,Glx) -Val-Cys-Lys- (Thr,Gly,Ala,LeU2) 35). The thiols in the yeast enzyme, like those in lipoamide dehydrogenase, are relatively unreactive. Reactivity with phenyl mercuric acetate 29) and with A-ethylmaleimide 258) have been reported it is of interest that the 2-electron-reduced enzyme is stable in the presence of N-ethylmaleimide and excess NADPH 258), but four-electron reduction results in the presence of p-chloromercuriphenyl sulfonate 29). 

In vitro absorption was examined with the human colonic epithelial cell line NCM460 (Said et al. 2000). The presented results are similar to those observed for Caco-2 cells thus active transport (Km = 0.14 pM) is inhibited by structural analogues (lumiflavin and lumichrome), metabolic inhibitors i.e. temperature, sodium azide, DNP) and sulfhydryl group modifying p-chloromercuriphenyl sulfonate (p-CMPS). Studies have demonstrated Na" -independency, energy-dependency and regulation by the activity of Ca " /calmodulin kinase (Tables 36.1 36.4). An adaptive regulation under conditions of deficiency and oversupplementation has also been noted. 

Rothstein A, Mack E (1991) Actions of mercurials on cell volume regulation of dissociated MDCK cells. Am J Physiol 260 C113-C121 Schaeffer JF, Preston RL, Curran PF (1973) Inhibition of amino acid transport in rabbit intestine by P-chloromercuriphenyl sulfonic acid. J Gen Physiol 672 131-146... 

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