Diet cola

In this present work, an alternative PLS-2 method was investigated and applied to the determination of sodium benzoate (itsed for preservatives) artificial sweeteners Aspartame, Acesitlfame-K and caffeine in diet cola drinks. 

The diet cola drinks were degassed 10 min in an ultrasonic bath and diluted 60-fold in 0.1 N HjPO. In 10 mL volumetric flasks, an aliquot (0.35-0.4 mL) of the pretreated sample of cola drink was introduced, and then diluted to the mark with 0.1 N HjPO. The electronic absorption spectrum was recorded from 190-300 nm against a blank of 0.1 N H PO. ... 

Figure 8.10—Electrophoretic separation of three species a, h and c. in this example, the electro-osmotic flow moving towards the cathode carries all charged and neutral species. Negatively charged species, although attracted towards the positive pole (or anode) cannot overcome the electro-osmotic flow and thus move towards the cathode. Separation of caffeine (c) from aspartame (a-) and benzoate (b ) in a Diet Cola sample. The presentation of data is in the form of a 3-D electrophoregram. (Reproduced by permission of TSP.)...

Soft drinks (e.g., ginger ale, diet cola) 0.103-2.084 Derived from Schenk et al. 1989... 

In the most recent study at the time adverse effects were detected in animals at a dietary level of 3 per cent and above, and so a NOAEL was set at a level of i per cent in the diet. This is equivalent to 500 mg of saccharin per kg of body weight, which in turn is equivalent to a person drinking about 200 cans of diet cola a day The acceptable daily intake (ADI) is determined by dividing the NOAEL by a safety factor. The safety factor used is usually 100. 

By extrapolation from the dose-response information in rats, it has been estimated that a o.oi per cent dietary level of saccharin, equivalent to about two cans of diet cola a day would lead to a lifetime risk of bladder tumours of 2.5 X 0.0000000001 (2.5 X io °) or 2.5 in ten thousand million This does not take into account the fact that (i) the bladder tumours occurred only in male rats treated in a particular way (2) the doses given were unrealistically high and (3) there is no human evidence for the effect. Thus even this estimate is erring very much on the side of caution. 

Finally, as a reminder that stabiUty studies are not restricted to pharmaceutical products, a chromatogram taken from the study of a stability study of LL-aspartame (NutrasweeO in diet cola is shown in Figure 12.14. The same conditions were used for the resolution of the stereoisomers of aspartame (Figure 12.6), and the study of the stability of LL-aspartame in distilled water and coffee sweetened with Nutrasweet. ... 

Figure 12.14 Determination of L,L-aspartame and its decomposition products in a diet cola. 1 L,L-uspartame, 6 a diketopiperazine degradation product and C caffeine. Conditions are as for Figure 12.6. (Motellier and Wainer, 1990.)...

Additives to diet cola—caffeine, saccharin, and sodium benzoate—represent several different classes of compounds that may be extracted on anion-exchange sorbents simultaneously. Caffeine and saccharin are neutral compounds and sodium benzoate is an organic anion (Fig. 6.14). Caffeine and saccharin sorb by van der Waals interaction on the silica-bonded ion-exchange sorbent and the sodium benzoate by anion exchange. The sorbent chosen is a strong anion exchange in chloride form. The sorbent is first cleaned and... 

Diet cola (10 mL), no sample additions, de-gas sample by shaking. Caffeine, saccharin, and sodium benzoate. 

Bakerbond Application Note FF-006 Extraction of Caffeine, Saccharin, and Sodium Benzoate from Diet Cola. Published with the permission of J. T. Baker, Inc. (see Suggested Reading, Chapter 1). 

Caffeine is found in coffee, tea, chocolate, and cola drinks, which includes both diet cola drinks (unless specified that it is caffeine-free) and regular cola drinks. It is also present in related plant products like the cola (kola nut (ref.l), and guarana (ref.2). Concentrated dosages are available in liquid, tablet, and suppository form and in certain medications/herbal preparations. 

Figure 7.21. Isocratic (A) and gradient (B) separations of a diet cola sample. Diagram courtesy of Dionex Corporation.

Figure 8.10 Electro-kinetic separation of three species a, and c. Left, The electro-osmotic flow carrying all of the charged or neutral species along with it, is directed towards the cathode. The negative species though attracted hy the positive pole cannot overcome the electro-osmotic flow and are therefore displaced towards the cathode. Right, separation of caffeine c and of the anions of aspartame a and of henzoate h from a sample of DIET COLA. Presentation in the form of a 3D electropherogram (reproduced courtesy of TSP).

Soft drinks are about as dense as water (1.0 g/cm ) many common metals, including iron, copper, and silver, have densities around 9.5 g/cm. (a) What is the mass of the liquid in a standard 12-oz bottle of diet cola (b) What is the mass of a dime (Hint A stack of five dimes has a volume of about 1 cm. )... 

Exacerbation of hemochromatosis (an inherited disease in which excessive iron accumulates in the body) was reported in a 68-year-old woman with a history of type 2 diabetes, asthma, hypothyroidism, borderline hypertension, borderline diastolic dysfunction, and a fatty liver. The women had been taking 200 mg of milk thistle daily for over a year, along with two extra-strength acetaminophen pills every 2 to 3 days, and a can of cola (presumably a diet cola) every day (Whittington 2007). A letter regarding this case noted that elevated liver enzymes, reported as a sign of hemochromatosis exacerbation, may occur with regular acetaminophen use (Kidd 2008). 

The master production schedule (MPS) is the detailed production plan for the end items to be produced by the plant or firm. It is constrained by the production plan. For example, if the production plan calls for 1,000 two-liter size bottles of soda to be produced in January, then the MPS is the detailed plan to produce all the different types of two-liter size bottles of soda produced in January. This may mean that 250 two-liter bottles of cherry cola are produced in week 1 250 bottles of diet cola in week 2 250 bottles of sugar cola in week... 

Metrosep A Supp 1 HS is a special separator, which has been developed for rapid analysis of orthophosphate in cola soft drinks. Its short length of 50 mm and very low ion-exchange capacity also allow rapid analysis of some common inorganic anions in weakly contaminated sample matrices. The corresponding hyperbranched polymer, the lonPac Fast Anion IIIA (see Section 3.4.3), which has been developed for the same application, is more robust due to its chemistry and standard length of 250 mm. Moreover, it elutes citrate faster and can, therefore, be applied for analyzing diet cola drinks, too. 

Figure 3.76 Fast analysis of orthophosphate and citrate in a diet cola soft drink. Separator column lonPac Fast Anion IIIA column dimensions 250 mm x 3 mm i.d. column temperature 30 °C eluent 22 mmol/L KOH (EG) ...

Figure 10.230 Separation of inorganic cations in a diet cola soft drink. Separator column lonPac CS12A with guard eluent methanesul-fonic acid gradient 16mmol/L isocratic for 5 min and then step to 40 mmol/L for 5 min flow rate 1 mlVmin detection suppressed...

Figure 5 Purge-and-trap analysis of 5 ml of a 1/10 dilution of diet cola beverage.

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