Diagnostic enzymes

Diagnostic enzyme assay that uses stable-isotope-labeled substrates to detect L-argi-nine glycine amidinotransferase deficiency. Clin Chem 49 803-805... 

Molded Dry Chemistry. In general, most enzymes are very fragile and sensitive to pH. solvent, and elevated lemperaiurts. The catalytic activity of most enzymes i> reduced dramatically ils the temperature is increased, Typi cal properties of diagnostic enzymes are given in Table 1. t he presence of ionic salts and other chemicals can considerably influence enzyme stability. To keep or sustain enzymatic activity, the redox centers must remain intact. The bulk of the enzyme, polymeric in composition, is an insulaior. thus. altering ii does not reduce the enzyme s catalytic activity, li... 

Prince HE. Evaluation of the INOVA diagnostics enzyme-linked immunosorbent assay kits for measuring serum immunoglobulin G (IgG) and IgA to deamidated gliadin peptides. Clin Vaccine Immunol 2006 13 150-151. 

Tab. 5.4 Seven essential hepatocellular and four biliary diagnostic enzymes together with their respective terminology, abbreviations, EC code and specificity (+ + + + )...

Anhydro-D-glucitol (AG, 164 g/mol) is one of the main sugar alcohols in human cerebrospinal fluid and blood. In plasma, the normal AG level is 24.6 7.2 mg/L (539 patients tested), while patients with diabetes mellitus show reduced AG levels of 7.3 7.1 mg/L (808 patients tested). Because reduced AG levels are specific indicators of this type of diabetes, a diagnostic enzyme assay for AG has been developed. The assay employs the enzymes... 

Warnick GR, Spain M, Kloepfer H, Volke TM. Standardization of a commercial (Boehringer Mannheim diagnostics) enzymic method for cholesterol. Cfin Chem 1989 35 409-13. 

Bt and from conventional insecticides. As avidin is a natural food protein found in eggs, it may be easier to register than other insecticide proteins that are not present in the food supply [352]. Beta-glucuro-nidase was also produced in corn seed by ProdiGene for use as a diagnostic enzyme, but it is not commercially available [285, 286]. 

Clampitt, R. B., and R. J. Hart. 1978. The tissue activities of some diagnostic enzymes in ten mammalian species. Journal of Comparative Pathology 88 607-621. 

Enzymes are polymeric in nature and very specific catalysts. They are extensively used in diagnostics, immunodiagnostics, and biosensors to measure or amplify signals of many, but specific, metabolites. Typical properties of select diagnostic enzymes are presented in Table III. 

Table in. Typical Properties of Sdect Diagnostic Enzymes... 

Murooka Y, Yamashita M (2001) Genetic and protein engineering of diagnostic enzymes, cholesterol oxidase and xylitol oxidase. J Biosci Bioeng 91 433-441... 

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