Detergent resistant membrane

FIGURE 10.12 The mole ratio of carotenoid/phospholipid and carotenoid/total lipid (phospholipid + cholesterol) in raft domain (detergent-resistant membrane, DRM) and bulk domain (detergent-soluble membrane, DSM) isolated from membranes made of raft-forming mixture (equimolar ternary mixture of dioleoyl-PC (DOPC)/sphingomyelin/cholesterol) with 1 mol% lutein (LUT), zeaxanthin (ZEA), P-cryptoxanthin (P-CXT), or P-carotene (P-CAR). 

Brown, D.A., London, E. Structure of detergent-resistant membrane domains does phase separation occur in biological membranes Biochem. Biophys. Res. Commun. 1997, 240, 1-7. 

Rakheja, D. et al. (2004). CLN3P, the Batten disease protein, localizes to membrane lipid rafts (detergent-resistant membranes). Biochem. Biophys. Res. Commun. 317(4), 988-991. 

LIPH) RAFT PROTEOMICS MORE THAN JUST DETERGENT-RESISTANT MEMBRANES... 

Lipid raft proteomics more than just detergent-resistant membranes... 

Bomer, G.H., Sherrier, D.J., Weimar, T, Michaelson, L.V., Hawkins, N.D., Macaskill, A., Napier, J.A., Beale, M.H., Lilley, K.S. and Dupree, P. (2005) Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts. Plant Physiol. 137, 104-116. 

Nebl, T., Pestonjamasp, K.N., Leszyk, J.D., Crowley, J.L., Oh, S.W. and Luna, E.J. (2002) Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes. J. Biol. Chem. 

Lichtenberg D, Goni FM, Heerklotz H. Detergent-resistant membranes should not be identified with membrane rafts. Trends Biochem. Sci. 2005 30 430-436. 

WaUach DF, Schmidt-Ullrich R. Isolation of plasma membrane vesicles from animal cells. Methods Cell Biol. 1977 15 235-276. Brown DA. Lipid rafts, detergent-resistant membranes, and raft targeting signals. Physiology 2006 21 430-439. 

Sheets ED, Holowka D, Baird B. Critical role for cholesterol in Lyn-mediated tyrosine phosphorylation of FceRI and their association with detergent-resistant membranes. J. Cell Biol. 1999 145 877-887. 

Goud B, Salamero J, Johannes L. Targeting of Shiga toxin b- 28. subunit to retrograde transport route in association with detergent-resistant membranes. Mol. Biol. Cell 2001 12 2453-2468. 

Brown DA. Lipid rafts, detergent-resistant membranes, and rafttargeting signals. Physiology 2006 21 430-439. 

Both mPA-PLAjU and mPA-PLAj/ , like many phosphate-recognizing enzymes such as phosphatase and ATPase [40], are inhibited by vanadate [51]. When expressed in insect Sf9 cells, neither mPA-PLAja nor mPA-PLAj/ were secreted. Both were recovered from the Triton X-lOO-insoluble fraction of the cell membrane. An immunofluorescence analysis confirmed that these proteins are localized exclusively to the plasma membrane. Both mPA-PLAja and -fi may locahze in the detergent-resistant membrane domain, referred to as raft structures, and function as lysoPA-producers under certain conditions [51]. 

PLD activation and produce 2-acyl-lysoPA in detergent-resistant membrane. LPA3/EDG7 is specifically activated by 2-acyl-l-lysoPA. 

Wolf Z, Orso E, Werner T, et al. (2007) Monocyte cholesterol homeostasis correlates with the presence of detergent resistant membrane microdomains. Cytometry A 71 486 94... 

Lamaze C, Dujeancourt A, Baba T et al. (2001). Interleukin-2 receptors and detergent-resistant membrane domains define a clathrin-independent endocytic pathway. Mol Cell, 7, 661-671. 

Swinnen, J. V., Van Veldhoven, P. P., Timmermans, L., De Schrijver, E., Brussehnans, K., Vanderhoydonc, F., Van de Saiide, T., Heemers, H., Heyns, W. and Verhoeven, G., Fatty acid synthase drives the synthesis of phospholipids partitioning into detergent-resistant membrane microdomains, Biochem Biophys Res Commun 302 (2003) 898-903. 

Smith D.C., Sillence D.J., Falguieres T., Jarvis R.M., Johannes L., Lord J.M., Platt F.M., Roberts L.M., The association of Shiga-like toxin with detergent-resistant membranes is modulated by glucosylceramide and is an essential requirement in the endoplasmic reticulum for a cytotoxic effect. Molecular Biology of the Cell 17 (2006) 1375-1387. 

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