Detection by Fourier-transform Infrared Spectroscopy Carbonyl Metalloimmuno Assay, CMIA

Detection by Fourier-transform Infrared Spectroscopy (Carbonyl Metalloimmuno Assay, CMIA) 

Carbonyl metalloimmunoassay (CMIA) is a heterogeneous competitive-type metalloimmunoassay using metal carbonyl complexes as tracers, organic solvents (ethyl acetate, isopropyl ether) to separate the free and bound fractions, and Fourier-transform infrared (FT-IR) spectroscopy for quantification. The choice of FT-IR 

The use of infrared spectrometry for quantitive analysis became possible only in the 1980s, when affordable and user-friendly benchtop Fourier-transform spectrometers became available. The sensitivity of the FT-IR spectroscopy was, however, insufficient to meet the requirements of the immunoassay. To address this problem, an instrument equipped with a liquid nitrogen-cooled detector made from a semi-conducting material, for example MCT (mercury-cadmium-telluride) or InSb (indium antimonide), was used to increase sensitivity by a factor of 20 compared with the thermal detector DTGS found in standard FT-IR machines. Use of a light-pipe cell with a long optical path (20 mm) for a 

Expansion of the v(CO) region between 2200 and 1900 cm Inset Beer s law plot for 27 ranging from 1.25 to 30 pmol/tube. Absorbance at 2057 cm  

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