Deoxyribonucleic acid antibody specificity

Wood et al. (1991) have used the Southern hybridization method for detecting DNA amplification and a possible structural rearrangement of the HER-2/nen oncogene in 1 of 12 bladder tumors. Amplification of this oncogene in the tumor was sixfold that of oncogene found in placental DNA. Approximately 36% of the tumors studied overexpressed HER-2 mRNA, which was 3- to 38-fold that of normal urothelium. HER-2 overexpression occurred in superficial and invasive tumors. Deoxyribonucleic acid amplification occurs infrequently in bladder carcinoma, in contrast to its occurrence in some other carcinomas. Immunohistochemical analysis has shown that pi85 HER-2 polyclonal antibody is specific for HER-2 protein overexpression in bladder carcinoma. This study was carried out prior to the use of Herceptin. 

There is an ongoing requirement in cell and molecular biology for the preparation of antibodies to use as probes for specific proteins. Two main strategies exist to raise appropriate antibodies. A complimentary deoxyribonucleic acid (cDNA), or gene sequence encoding the protein of interest can be expressed in a heterologous species,... 

Butler, V. P., Beiser, S. M., Erlanger, B. F., Tanenbaum, S. W., Cohen, S., Bendich, a. Purine-specific antibodies which react with deoxyribonucleic acid Proc. nat. Acad. Sci. (Wash.) 48, 1597-I6O2 (1962). 

Levine, L., Murakami, W. T., Van Vunakis, H., Grossman, L. Specific antibodies to thermally denatured deoxyribonucleic acid of phage T4. Proc. nat. Acad. Sci. (Wash.) 46, 1038-1043 (i960). 

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