Deoxyribonucleic acid denaturation

Immunocapture-polymerase chain reaction (IC-PCR) is a synthesis of two commonly used diagnostic tools. This method exploits the high-affinity binding of antibodies to provide a facile method of purification, usually from a complex matrix, supplying the substrate for PCR detection. PCR exponentially amplifies a deoxyribonucleic acid (DNA) template in a temperature-dependent fashion by the annealing of oligonucleotide primers, enzymatic extension of bound primers by a heat-stable polymerase, followed by denaturation of... 

In in vitro studies there is little observable reaction between 0s04 and native deoxyribonucleic acid (DNA), but it does react with denatured DNA, in which the thymine bases have probably been hydroxylated to 5,6-dihydroxy thymine via an osmium(VI) ester.610... 

Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 1962 5 109-118. 

Delocalization energy, 374. See also Resonance energy Denaturation of ethanol, 581 of proteins, 1087 Dendrolasin, 1046 Deoxyribonucleic acid (DNA)... 

Bernardi G. (1962). Chromatography of denatured deoxyribonucleic acid on calcium phosphate. Biochem. J. 83 32-33. 

Eigner J. and Doty P. (1965). The native, denatured and renatured states of deoxyribonucleic acid. J. Mol. Biol. 12 549-580. 

Perey DYE, Cooper MD, Good RA (1968) Lymphoepithelial tissues of the intestine and differentiation of antibody production. Science 161 265-266 Plescia OJ, Braun W, Palczuk NC (1964) Production of antibodies to denatured deoxyribonucleic acid (DNA). Proc Natl Acad Sci USA 54 1281-1285 Plescia OJ, Palczuk NC, Cora-Figueroa E, Mukheijee A, Braun W (1965 a) Production of antibodies to soluble RNA. Proc Natl Acad Sci USA 54 1281-1285 Plescia OJ, Palczuk NC, Braun W, Cora-Figueroa E (1965 b) Antibodies to DNA and a synthetic polydecaribonucleotide produced by oligodeoxyribonucleotides. Science 148 1102-1103... 

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