Deoxyribonuclease assay

Enzyme activity has been sought in seawater Strickland and Solorzano [288] looked for photomono esterase activity, while Maeda and Taga [289] used a flu-orometric method for assaying deoxyribonuclease activity in both seawater and sediment samples. 

Twenty-seven out of 44 FDA-approved biopharmaceuticals have been tested in a battery of genotoxicity assays. Eighty-five different assays performed yielded negative results. The most commonly performed assays were the Ames test, the chromosomal aberration assay in human lymphocytes, the mouse lymphoma gene mutation assay, and the mammalian in vivo erythrocyte micronucleus test. Examples of the range of biopharmaceutical products tested include, domase alfa (deoxyribonuclease I-DNAse), trastuzumab (mAb to human epidermal growth factor receptor 2), alteplase (tissue plasminogen activator), infliximab (mAb to the human tumor necrosis factor a). 

Blikstad I, Markey F, Carlsson L, etal. (1978) Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I. In Cell 15 935-43... 

Assay of Deoxyribonuclease. Hydrolysis of DNA is followed by methods similar to those used with RNA. Another unexpected optical shift occurs, an increase in density at 260 mu occurs on hydrolysis this may be caused by rupture of hydrogen bonds involving the bases. 

Pardee and co-workers (225,225a,227,227a) have investigated the activity of 9 bacterial enzymes after infection of E. coli with various T phages, i.e., apyrase, ribonuclease, deoxyribonuclease, alkaline and acid proteases, pyruvic oxidase, formic dehydrogenase, serine deaminase, and catalase. The difficulties of assay of labile enzymes in the broken cell systems are indicated by the early report of an increase in apyrase activity (225) which later was attributed to a technical defect in the... 

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