Density gradient centrifugation Percoll gradients

The molar ratio of the components of metaphase chromosome was determined by Uchiyama and his colleagues (Uchiyama et al., 2005). Metaphase chromosomes has been isolated from synchronized human cell line and then purified by sucrose density gradient centrifugation and Percoll density gradient centrifugation. The major components described in this chapter are summarized here. Molar ratio is provided per 100 histone H4 molecules. 

The cytoplasmic organelles may be separated from each other by differential centrifugation or by density gradient centrifugation using sucrose or one of the commercially available alternatives, e.g. Percoll or Metrizamide. Some alternative approaches are considered by Howell et al. (1989). 

Murphy, M.A. and Herscowitz, H.B. (1984). Heterogeneity among alveolar macrophages in humoral and cell-mediated immune responses separation of functional subpopulations by density gradient centrifugation on percoll. J. Leuk. Biol. 35, 39-54. 

Subcellular fractions were prepared by standard differential and density gradient centrifugation protocols. Purified fractions of mitochondria and peroxisomes were prepared by Percoll and Nycodenz centrifugation, respectively. The following marker... 

Although these hPT cells are predominantly (>85%) of PT origin, a small degree of contamination with other epithelial cell populations is unavoidable and, therefore, will exist. While our laboratory has typically not performed additional purification procedures with the hPT cells, options are available to further enrich the PT cell preparation. For example, density gradient centrifugation of the renal cortical cells on Percoll can be performed to obtain PT cells that are >95% of PT origin and distal tubular (DT) cells that have minimal contamination with cells of the PT region, as we have done with cortical cells from rat kidneys (Lash and Tokarz, 1989 Lash et al., 1995). 

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