Dehydroepiandrosterone measurement

Corpechot C, Robel P, Axelson M, et al Characterization and measurement of dehydroepiandrosterone sulphate in the rat brain. Proc Natl Acad Sci USA 78 4704-4707, 1981... 

B4. Benbassat, C. A., Maki, K. C., and Unterman, T. G., Circulating levels of insulin-like growth factor (IGF) binding protein-1 and -3 in aging men Relationships to insulin, glucose, IGF, and dehydroepiandrosterone sulfate levels and anthropometric measures. J. Clin. Endocrinol. Metab. 82, 1484-1491 (1997). 

More recently, the activity of dehydroepiandrosterone sulphotransferase, a measure of SULT2A/2B activity, has been shown to be reduced in patients with primary biliary cirrhosis and alcoholic cirrhosis [86]. 

Rosenfield RL. A competitive protein binding method for the measurement of unconjugated and sulfate-conjugated dehydroepiandrosterone in peripheral plasma. Steroids 1971 17 689-96. 

Chadwick, C.A. Owen, L.J. Keevil, B.G. Development of a method for the measurement of dehydroepiandrosterone sulphate by liquid chromatography-tandem mass spectrometry. Ann. CUn. Biochem. 2005, 42 (Pt 6), 468-474. 

Gas Chromatographic Procedure for the Measurement of Pregnanediol, Androsterone, Etiocholanolone, and Dehydroepiandrosterone Biochem. Women Methods Clin. Invest. 1974 151-179 CA 82 135072p... 

Dehydroepiandrosterone is the principal 17-ketosteroid of the 3/3-hy-droxy series present in urine. The measurement of this fraction has been given certain diagnostic significance. For this reason it is particularly important that conditions of hydrolysis be chosen which give maximum conversion of dehydroepiandrosterone sulfate to dehydroepiandrosterone. If this is not the case, digitonin precipitation (Section VIII,2) will give erroneously low results and colorimetric assays could be in error as a result of differences in extinction coefficients of the transformation products present. 

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