Dehydroepiandrosterone sulfate measurement

B4. Benbassat, C. A., Maki, K. C., and Unterman, T. G., Circulating levels of insulin-like growth factor (IGF) binding protein-1 and -3 in aging men Relationships to insulin, glucose, IGF, and dehydroepiandrosterone sulfate levels and anthropometric measures. J. Clin. Endocrinol. Metab. 82, 1484-1491 (1997). 

Dehydroepiandrosterone is the principal 17-ketosteroid of the 3/3-hy-droxy series present in urine. The measurement of this fraction has been given certain diagnostic significance. For this reason it is particularly important that conditions of hydrolysis be chosen which give maximum conversion of dehydroepiandrosterone sulfate to dehydroepiandrosterone. If this is not the case, digitonin precipitation (Section VIII,2) will give erroneously low results and colorimetric assays could be in error as a result of differences in extinction coefficients of the transformation products present. 

Rosenfield RL. A competitive protein binding method for the measurement of unconjugated and sulfate-conjugated dehydroepiandrosterone in peripheral plasma. Steroids 1971 17 689-96. 

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