Datura aurea

Robins, R. J., Parr, A. J., Payne, J., Walton, N. J. and Rhodes, M. J. C. 1990. Factors regulating tropane alkaloid production in a transformed root culture of a Datura Candida x Datura aurea hybrid. Planta, 181 414-422. 

FOLIA DATURAE QUASI OLIA AUREA — Leaves like Gold-leaf... 

Hairy roots are not as readily manipulated by altering culture conditions or pH as are suspension cultures. However, the effect of temperature on growth and hyoscyamine production in transformed root cultures of Datura stramonium has been demonstrated by Hilton and Rhodes [86]. Another way to enhance the secondary metabolite accumulation of hairy roots is the addition of precursors and/or metabolic intermediates to the growth medium. The addition of (R,S)- phenyllactic acid increased significantly the accumulation of hyoscyamine and scopolamine in the hairy root culture of Datura Candida x D. aurea [72]. 

Alkaloids of a Datura Candida cultivar, D. aurea and various hybrids Fitoterapia 61(2) 148-152. 

Hyoscyamine 6P-hydroxylase (H6H, EC 1.14.11.11) is a 2-oxoglutarate-dependent dioxygenase and is the final enzyme of the tropane alkaloid biosynthetic pathway. The H6H catalyzes the conversion of hyoscyamine into 6P-hydoxyhyoscyamine and scopolamine by two sequential reactions [24, 25]. Firstly, it catalyses the 6P-hydroxylation of hyoscyamine and secondly, the formation of the epoxy group leading to scopolamine [13]. Several works have revealed that the epoxidation activity is much lower than the hydroxylation one [11, 26]. These findings are in agreement to those reported in our laboratory about the H6H enzyme isolated from Brugmansia Candida (syn. Datura Candida) [27-29]. This South America n native plant is a natural hybrid between B. aurea and B. versicolor and a tropane alkaloids producer [30,31]. 

Datura comigera Hook. Brugmansia aurea Lageeih. sub nom. D. aurea (Lagerh.) Safe. 

Hartmann et al. 1986), respectively. To date, 3a-phenylacetoxytropane was found again only in transformed root cultures of a Brugmansia Candida x aurea hybrid (Robins et al. 1990) as well as of Hyoscyamus x gyGrjfyi (lonkova et al. 1994), whereas the occurrence of 3a-cinnamoyloxytropane is confined to L pubiflora. A stereochemically not determined 3-phenylacetoxy-6,7-epoxytropane, found in the seeds of Datura ferox, has been mentioned already above (Vitale et al. 1995). 

Solandra (4), Brugmansia (3), Datura (5), Physalis (1)]. Surprisingly, 3P-acetoxytropane was identified only in a few species of the Solanoideae (H. albus, H. pusiUus, B. Candida x aurea, D. wrightii, P. peruviana). Finally, 3p-(2-methylbutyryloxy)tropane seems to be confined to D. inoxia (Witte et al. 1987). 

---