Darkfield imaging

Figure 15.13. Combined imaging and therapy of SKBR-3 cells using anti-HER2 coated nanoshells. Darkfield imaging (top row), calcein AM staining for cell viability (middle row), and silver staining of nanoshell binding (bottom row). Increased contrast was observed only when cells were incubated with anti-HER2 nanoshells, and only these samples experienced cell death after exposure to a NIR laser. Reprinted with permission from Ref. 19. Copyright 2005 American Chemical Society.

Figure 20.30. Darkfield image of unstained catalyst later (Pt shown in white) (unpubhshed...

Light microscopy allows, in comparison to other microscopic methods, quick, contact-free and non-destmctive access to the stmctures of materials, their surfaces and to dimensions and details of objects in the lateral size range down to about 0.2 pm. A variety of microscopes with different imaging and illumination systems has been constmcted and is conunercially available in order to satisfy special requirements. These include stereo, darkfield, polarization, phase contrast and fluorescence microscopes. 

Fig. 1. Darkfield illumination the image is formed by light scattered from the specimen detail against a dark field.

Darkfield (DF) imaging was performed with the tilted beam technique, the reflection selected by a 6 x 10 3 rad.objective aperture. 

Figure 4.7 (A) Schematic illustration of fluorescence enhancement experiment Ag nanoprisms are adsorbed on top of monolayer of Rhodamine red on glass slide. (B) Darkfield scattering image of an area of the substrate. Each of colored...

Unlike darkfield microscopy, which is primarily used for imaging at the cell-surface level, OCT is an imaging modality that can be used to acquire optical images of samples at different depths, up to several millimeters with sub-micrometer resolution. OCT works similarly to ultrasound, but utilizes the reflections of light through tissue to generate images rather than reflections of sound waves. 

Figure I. CCD images of homogeneous color populations of individual metallic particles (left side) 60 nm diameter silver particles and (right side) 60 nm diameter gold particles, as viewed using an optical microscope configured for darkfield illumination with a 50 X objective lens.

---