Cytokines delivery vector

LAB as cytokine delivery vector It is now recognized that regulatory T cells (Treg) play a central role in gut homeostasis by maintaining immune tolerance and controlling inflammation. Indeed, a h) per-activation of CD4(+) T helper cells leads to an excessive and uncontrolled immune response, such as in the case of CD and UC, which have been putatively described to possess the Th 1/Th 17 and Th2 phenotype respectively (Ruddy et al. 2004 Leon et al. 2006). Several studies have shown that certain profiles of cytokines, which are produced by immune cells and involved in T cell activation and differentiation, have been directly associated with IBD. In particular, cytokines such as IFTSf- y, IL-17, IL-22, and IL-23 are dysregulated in IBD (Sarra et al. 2010). 

Cationic liposomes have also been used for treating neurodegenerative disease in in vivo models of Parkinson s disease and EAE. For example, EAE symptoms were inhibited by a single injection of therapeutic cytokine (IL-4, IFNp and TGF(3) lipoplexes directly into the CNS. Table 17.3 (below) summarizes gene delivery vectors used in MS. A list of cationic lipid/liposomes enabling in vivo and ex vivo transfection to the CNS is provided in Table 17.2. 

Pertmer, T. Robersts, T. Hynes, J. Influenza virus nucleo-protein-specific immunoglobulin G subclass and cytokine responses elicited by DNA vaccination are dependent on the route of vector DNA delivery. J. Virology 1996, 70, 6119-6125. 

Andrea W. Bledsoe, Cheryl A. Jackson, Sylvia McPherson and Casey D. Morrow, Cytokine production in motor neurons by poliovirus replicon vector gene delivery. Nature Biotechnology, 18 (2000), 964-969. 

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