Cuphea lanceolata

Bafor, M., Jonsson, L., Stobart, A.K. and Stymne, S. (1990a) Regulation of tricylglycerol biosynthesis in embryos and microsomal preparations from the developing seeds of Cuphea lanceolata. Biochem. J. Ill, 31-38. 

Webb, D.M., Knapp, S.J. and Tagliani, L.A. (1992) Restriction fragment length polymorphism and allozyme linkage map of Cuphea lanceolata. Theor. Appl. Genet. 83, 528-532. 

Schuch R, Brummel M, Spener F. 6-Ketoacyl-acyl carrier protein (ACP) synthase HI in Cuphea lanceolata seeds Identification and analysis of reaction products. J Plant Physiol 1994,143 556-560. 

DECANOYL-ACYL CARRIER PROTEIN INHIBITS THE B-KETOACYL-ACYL CARRIER PROTEIN SYNTHASE III IN CUPHEA LANCEOLATA SEED EXTRACT... 

Table 1. Variation of KAS Ill-activity in Cuphea lanceolata seed extract in the presence of reducing equivalents and butyryl-ACP...

Figure 1. Influence of acyl-ACPs on the activity of KAS III in Cuphea lanceolata seed extract. A, Activity of KAS III in the presence 0 pM ( ), 0.2 pM (, 0.5 pM (O), 1.0 pM and 2.0 pM (O) acyl-ACPs B, Elongation products of KAS III reaction using 10 pM acetyl-CoA (1), supplied with 10 pM butyryl-ACP (2), hexanoyl-ACP (3), octanoyl-ACP (4) and decanoyl-ACP (5). Bars represent elongation (%) of acetyl-CoA ( ) and acyl-ACPs (JM).

A comparison of deduced amino acid sequences from 10 plant acyl [Acyl Carrier Protein] thioesterases (TE) and their relationship to six Cuphea TEs is discussed. Expression studies of one TE from Cuphea lanceolata (C/TE13) in roots, flowers, leaves, maturing embryos and seeds showed a preferred expression in flowers. Heterologous expression in transgenic Brassica napus revealed 3 % capric acid in immature seeds of the T2 progeny. 

CLONING OF A cDNA CODING FOR A GLYCEROL-3-PHOSPHATE DEHYDROGENASE FROM CUPHEA LANCEOLATA... 

Figure 2 Dot-matrix plot of Cuphea lanceolata (C/GPDH20) versus mouse (MouGPDH) amino acid sequence of GPDH.

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