CRISPR

Strain Size Genes CRISPRs Scaffolds 16S rfiNAs 23S rRNAs 5S rRNAs tRNAs... 

C. heijerinckii NCIMB 8052 Wild type CRISPR NAP Cell survival pYW Cas-derivatives spoOA [36]... 

Haft, D.H., Selengut, ]., Mongodin, E.F., and Nelson, K.E. (2005) A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes. PLoS Comput. Biol, 1, e60. 

The rise and fall of CRISPRs - dynamics of spacer acquisition and loss. Mol. Microbiol, 85, 1021-1025. 

Bland, C., Ramsey, T.L., Sabree, F., Lowe, M. et al (2007) CRISPR Recognition Tool (CRT) a tool for automatic detection of clustered regularly interspaced palindromic repeats. BMC Bioinf, 8, 209. 

Rios, X. et al. (2013) Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res., 41, 4336 -4343,... 

F. et al (2013) RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat. Biotechnol., 31, 233—239. 

Oh, J.H. and van Pijkeren, J.P. (2014) CRISPR-Cas9-assisted recombineering in Lactobacillus reuteri. Nucleic Acids Res., 42, el31. 

K. et al. (2015) Markerless chromosomal gene deletion in Clostridium beijerinckii using CRISPR/Cas9 system. ). Biotechnol, 200, 1-5. 

B. et al. (2015) Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system. AppL Environ. Microbiol, 81, 2506—2514. 

Tong, Y., Charusanti, P, Zhang, L, Weber, T. et al (2015) CRISPR-Cas9 based engineering of actinomyc-etal genomes. ACS Synth. Biol, 4 (9), 1020-1029. 

E.R., Lundgren, M. et al. (2009) CRISPR-based adaptive and heritable immunity in prokaryotes. Trends Biochem. Sci,... 

Jakociunas, T., Jensen, M.K., and Keasling, J.D. (2016) CRISPR/Cas9 advances engineering of microbial cell factories. Metab. Eng., 34, 44-59. 

Terns, R.M. and Terns, M.P. (2014) CRISPR-based technologies prokaryotic defense weapons repurposed. Trends Genet, 30, 111 — 118. 

DNA targeting by the type I-G and type I-A CRISPR-Cas systems of Pyro-coccus furiosus. Nucleic Acids Res., 43, 10353-10363. 

TCA cycle 187,188 Crellox technique 240 CRISPR/Cas9 system 492 crocapeptins 466... 

Some DNA structures are unstable, being able to jump to other locations (deletion and insertion) or invert (discovered in plants in 1951 by Barbara McClintock) rediscovered in bacteria, insertion elements, transposons, homing nucleases, CRISPR, and so on, targeted and destabilized, particularly foreign, DNA. 

Qi, L.S. et al. (2013) Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell, 152 (5), 1173-1183. 

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