Cosmid vectors, gene cloning

Collins, J. Hohn, B. (1978). Cosmids a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage X heads. Proc. Natl. Acad. Sci. USA 75,4242-4246. 

Collins, J., and Hohn, B. (1978). Cosmids A Type of Plasmid Gene-Cloning Vector That Is Packageable in Vitro in Bacteriophage A Heads. Proc Natl Acad Sci 75 4242. 

Kuhstoss, S., M. Huber, J. R. Turner, J. W. Paschal, R.N. Rao (1996). A new shuttle cosmid vector, pKC505, for streptomycetes its use in the cloning of three different spiramycin-resistance genes from a Streptomyces ambofaciens library. Gene 183 231-241. 

For repetitive DNA sequences, such as alphoid repeats, especially for identification of centromeric regions on chromosomes, as well as in mterphase nuclei, I routinely prepare labeled probes by PCR using a centromeric DNA-specific set of primers. For single-copy gene mapping by FISH, it is essential to use DNA cloned into either cosmids or in yeast artificial chromosome vectors (YAC) m order to obtain the level of sensitivity that will enable visualization of signal with a fluorescence microscope... 

Slater et al. reported cloning and expression of A. eutrophus HI 6 PHB pathway in E. coli. Library was constructed in cosmid pVK 102. The study revealed the relation between PHB transcriptional control and transcriptional control in other regulations, and PHB biosynthetic pathway is controlled by nitrogen and oxygen limitation. Cosmid clone was subcloned and the PHB biosynthetic pathway gene with a fragment size of 5.2 kb was cloned into multicopy vectors, which can direct the PHB synthesis in E. coli to 80% dry weight. 

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