Correlative light and electron microscopy

Moran DT, Rowley JC. Biological specimen preparation for correlative light and electron microscopy, in Correlative Microscopy in Biology, Instrumentation and Methods (Hayat MA, ed.), Academic Press, New York, 1987, pp. 2-22. 

Powell, R. D., Halsey, C. M. R., and Hainfeld, J. F. (1998) Combined fluorescent and gold immunoprobes reagents and methods for correlative light and electron microscopy. Microsc. Res. Techn. 42,2-12. 

In this chapter we outline a general approach for the identification and localization of caimabinoid receptors within cells and tissues using correlative light and electron microscopy immunoperoxidase procedures. This approach, then, is followed by that using immunogold at the ultrastructural level, which can be applied to high-resolution locahzation of cannabinoid receptors. 

Giepmans, B. N., Deerinck, T. J., Smarr, B. L., Jones, Y. Z., and Ellisman, M. H. (2005) Correlated light and electron microscopy imaging of multiple endogenous proteins using quantum dots. Nat. Methods 2, 742-749. 

Svitkina, T. M., and Borisy, G. G. (1998). Correlative light and electron microscopy of the cytoskeleton of cnltnred cells. Methods Enzymol. 298, 570-592. 

As the issue of correlative light and electron microscopy (CLEM) has been very recently reviewed [141-145] in this section, we will very briefly consider the most consolidated possibilities that are offered to experimenters for a better implementation of ICC procedures in neurobiological studies. 

CLEM correlative light and electron microscopy, HRP horseradish peroxidase, ICC immunocytochemistry, LM light microscopy, NO nitric oxide, TEM transmission electron microscopy... 

Muller-Reichert T, Verkade P (2012) Introduction to correlative light and electron microscopy. Methods Cell Biol 111 17-29... 

Verkade P (2008) Moving EM the Rapid Transfer System as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing. J Microsc 230 317-328... 

Auinger S, Small JV (2008) Correlated light and electron microscopy of the cytoskeleton. Methods Cell Biol 88 257-272... 

Chatteijee et al., 1984 Sens et al., 1988), and cyclosporine (TrifiUis et al., 1984). Studies reported by Tay et al. (1988) in rabbit proximal tubule cultures with cisplatin revealed biochemical effects upon DNA synthetic activty that correlated with in vivo histochemical effects of this antitumor agent in animals. With respect to studies involving mercuric chloride and aminoglycoside antibiotics in primary renal cultures, light and electron microscopy revealed similar patterns of cellular pathology in vitro as compared to in vivo exposure in animals (Chatteijee et al., 1984 Aleo et al., 1987). 

Correlative microscopy involves multiple separate microscopy platforms, which are correlated to view the same exact structure of interest with complimentary information [10]. Such systems allow structure-function relationships to be determined at high resolutions. Examples of correlative microscopy include light and electron microscopy, light and atomic force microscopy, immunocytochemistry, and histochemistry, etc. 

In colloidal suspensions of anisotropic particles, the static structure factor plays a prominent role in particle size analysis. We have used transient electric birefringence (TEB) and electron microscopy, in addition to laser light scattering, to correlate our analysis of particle size distributions of bentonite suspensions. The complementary nature of TEB and photon correlation spectroscopy (PCS) in particle size analysis will be discussed. 

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