Probe copy screen

The screens below show the effects of selecting the other two options for copying Probe screens. The difference between the screens shown here may not be obvious because the screen captures are shown in gray scale. 

Both Probe screens and Oread Capture circuits can be copied and pasted into other documents. Here we will show how to paste them into a Microsoft Word document. We will start with the circuit of Part 2 on page 96 ... 

Copying screens from a Probe display is just as easy as it was to copy circuit drawings in Oread Capture. However, there are a few options in Probe for copying the screen that we can explore. Note in this section that your pasted screens will be slightly different than those shown here. This is because I have changed Probe to display plots with a white background to make the screen captures for this text easier to read. Your Probe screen displays plots with a black background. 

Here we have selected options to make the background transparent and use the screen colors shown in Probe. Click the OK button to copy the Probe screen to the clipboard. Switch to MS Word and paste the image into your document ... 

We can copy the data from several traces at the same time and then paste that data into a spreadsheet. In the Probe screen below, we have three traces displayed ... 

This duplex with mismatches can be transferred into E. coli just as any other plasmid, and there it will replicate. Half of the progeny plasmids will have the original DNA sequence, but the other half will be the result of copying the strand with the altered sequence, and so these progeny will code for the mutant protein. The mutant progeny can be identified by screening with a hybridization probe, and the bacteria harboring them can be used as a source of the mutant protein. 

Complementary DNAs (cDNA) are DNA copies of mRNAs. Reverse transcriptase is the RNA-directed DNA polymerase that synthesizes DNA strand, using purified mRNA as the template. DNA polymerase is then used to copy the DNA strand forming a double-stranded cDNA, which is cloned into a suitable vector. Once a cDNA derived from a particular gene has been identified, the cDNA becomes an effective probe for screening genomic libraries (Cowell and Austin, 1997). Annotated human cDNA sequences can be accessed from HUNT at http // www.hri.co.jp/HUNT (Yudate, 2001). 

Characterize retrotransposons. Once a positive clone has been identified, it can be used as a probe to isolate full-length copies of the element from a genomic library. These clones can also be characterized by phylogenetic screening. 

Gros You can take DNA from a particular cell population that you think is affected and screen with viral probes. You can look for the endogenous versus the extra copies that are replicated. 

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