Continuous-flow PCR

M. Kopp et al. 1998. Chemical Amplification Continuous-Flow PCR on a Chip. Science 280 1046-1048. 

Similar methods of flow-through PCR chips were also reported [212,907,948], Temperature analysis of continuous flow PCR has been carried out on both a glass-Si and glass-glass chip [949]. 

FIGURE 9.13 Schematic diagram of the microfabricated device for continuous-flow PCR and RT-PCR. There are two inlets for PCR (PCR1 and 2), two inlets for RT (RT1 and 2), and five outlets for product collection at 20, 25, 30, 35, and 40 cycles. The chip is placed over four heating blocks. Blocks a, b, and c are at appropriate temperatures for denaturation, extension, and annealing in PCR, respectively. Block d is for RT. The intersection between the RT and the PCR channels is denoted I [907]. Reprinted with permission from the American Chemical Society. 

Sun, K., Yamaguchi, A., Ishida, Y., Matsuo, S., Misawa, H., A heater-integrated transparent microchannel chip for continuous-flow PCR. Sensors Actuators B... 

Kopp, M.U., Luechinger, M.B., Manz, A., Continuous flow PCR on a chip. Micro Total Analysis Systems 98, Proceedings pTAS 98 Workshop, Banff, Canada, 13-16 Oct. 1998, 7-10. 

Kopp MU, Mello AJ, Manz A. Chemical amplification continuous-flow PCR on a chip. Science 1998 280 1046-1048. 

K. Sun, A. Yamaguchi, Y. Ishida, S. Matsuo, and H. Misawa, A heater-integrated transparent microchaimel chip for continuous flow PCR, Sensors and Actuators B Chemical, 84, 283 (2002). 

Keywords Continuous flow PCR DNA analysis DNA microarrays Genetic analysis Integrated microsystems Microcapillary electrophoresis Microfluidics Micro-PCR devices Solid-phase extraction... 

C. Gartner, R. Klemm, and H. Becker, Methods and instruments for continuous flow PCR on a chip, Proc. SPIE 6465, Microfluidics, BioMEMS, and Medical Microsystems, 6465, 646502, 2007. 

Continuous-flow PCR (Figure 4.19). In continuous-flow PCR (CF-PCR), the sample is funneled through a microchannel that repeatedly passes through three different reaction zones at individual well-defined temperature levels. 

Figure 4.19 Continuous-flow PCR three heating elements, three reaction zones, defined...

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