Integrated DNA Analysis Microsystems

Integration of PCR and CE was achieved on a COC chip for the detection of bacteria (e.g., E. coli and Salmonella typhimurium). PCR was conducted in a 29-nL chamber, which was temporarily sealed off by gel valves during PCR. The gel valve can withstand a hydrostatic pressure of 100 psi, which is higher than the water vapor pressure of 12 psi achieved in PCR up to 95°C. After PCR and 

Micro-PCR is normally achieved in PC, Si, and glass but not PDMS because of its porosity. However, with a parylene (or poly-para-xylene) coating, problems of bubble formation, sample evaporation, and even protein adsorption are solved. In addition, no BSA or PEG additive is needed in the PCR mixture for dynamic coating because the parylene coating (4.5 pm thick) is hydrophobic. This can be 

FIGURE 9.5 Sex determination from human genomic DNA using the PCR-CE microdevice. Top amplification from female DNA. Only the 157 bp peak representing amplification from the X chromosome is seen. Middle amplification from male DNA both the 157 bp X-chromosome and the 200 bp Y chromosome amplicons are observed. Bottom DNA sizing ladder for amplicon size reference, run separately on the same device [282]. Reprinted with permission from the Royal Society of Chemistry. 

---