Consensus motif, sequence recognition

The specificity profile of individual SH2 domains was determined in a series of studies employing degenerate phosphopeptide library screens [53, 98] from which two consensus motifs emerged for SH2 domain binding [52-55,99,100]. One group of SH2 domains preferentially binds to a pTyr-Glu-Glu-Ile motif that defines a generic recognition sequence ... 

Sphingomyelin signature motif A consensus motif conferring sphingomyelin recognition by transmembrane domains. The consensus sequence of the motif is (VxxTLxxlY). Discovered for the first time in the COPI machinery protein p24. 

The Ras superfamily of GTP-binding proteins is composed of several subfamilies [7] which all contain the Ras-like domain of approximately 160 amino acids and 5 consensus sequences. Two of these highly conserved motifs are responsible for specific recognition of the guanosine nucleotide, and three are necessary for binding of the phosphate groups and complexation of a Mg++ ion, which is found in all Ras-like proteins. 

Figure 6. Multi-site phosphorylation of Sicl is required for recognition by Cdc4. The Cdk consensus phosphorylation site sequence is contrasted with the Cdc4 Phospho-Degron (CPD) consensus sequence. < > indicates disfavoured residues in the CPD motif.

Sources Pinna, L.A. Ruzzene, M.H. (1996) How do protein kinases recognize their substrates Biochim. Biophys. Acta 1314, 191-225 Kemp, B.E. Pearson, R.B. (1990) Protein kinase recognition sequence motifs. Trends Biochem. Sc/. 15, 342-346 Kennelly, RJ, Krebs, E.G. (1991) Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases, J. Biol. Chem. 266, 15,555-15,558. 

The sequence and structural conservations at the P 2, Po> Pi positions define a major TRAF2 binding motif that bears the consensus sequence of px(Q/E)E, in which Pro is shown in lower case because it can be substituted for other medium sized non-charged residues. Most of the binding sequences identified so far for TRAFl, 2, 3, and 5 are consistent with the motif, thereby explaining the recognition of diverse receptor sequences by TRAF2 (Fig. 6). 

The RNP motif is also referred to as RNP consensus sequence (RNP-CS), RNA binding domain (RBD), or RNA recognition... 

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