Consensus motifs

The conversion of a [3Fe-4S] into a [4Fe-4S] center was achieved by restoring the second residue of the consensus motif in E. coli fu-marate reductase (181) and in D. africanus ferredoxin III (161). However, the coordination scheme of the iron-sulfur centers of A. vinelan-... 

The ATPase activity of FhuC was originally claimed on the basis of the presence of the Walker A and Walker B consensus motif si and the location of FhuC... 

The specificity profile of individual SH2 domains was determined in a series of studies employing degenerate phosphopeptide library screens [53, 98] from which two consensus motifs emerged for SH2 domain binding [52-55,99,100]. One group of SH2 domains preferentially binds to a pTyr-Glu-Glu-Ile motif that defines a generic recognition sequence ... 

Jackson, M.R., Nilsson, T, and Peterson, P.A., 1990, Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum. EMBO J. 9 3153-3162. 

Is there a specific consensus site for SUMO attachment in substrate proteins. With studies on just a handful of substrates such as RanGAPl, I/tBa, p53, and c-jun the SUMO-acceptor site was found to be tPKxE (where tp is an aliphatic branched amino acid and x is any amino acid). The identification of a sumoylation consensus site is noteworthy because no such site for ubiquitination is found. Because ubiquitination is vastly more complex with hundreds of ligases, a common consensus sequence for ubiquitination may not exist. It must be pointed out, however, that the recent identification of the TEK box motif for APC suggests that consensus motifs for ubiquitin-acceptor sites might be found at least for some ligases. 

The rat brain and macrophage NOS isoforms are soluble, dimeric enzymes, each comprised of two identical subunits (Stuehr et al., 1991a Schmidt et al., 1991). Denatured molecular weights for the various NOS isoforms range from 130 to 150 kDa. The endothelium NOS is of unknown quaternary composition, and localizes primarily in the membrane fraction (Pollock et al., 1991), due to posttranslational myristoylation near the N-terminus (Lamas etaL, 1992). Phosphorylation consensus motifs are present on the brain and endothelial isoforms, with phosphorylation possibly down regulating NOS activity or preventing localization onto the membrane (Bredt et al., 1992 Michel et al., 1993). 

Sporns O, Jenkinson S (1997) Potassium ion- and nitric oxide-induced exocytosis from populations of hippocampal synapses during synaptic maturation in vitro. Neuroscience 80 1057-73 Stamler JS, Toone EJ, Lipton SA, Sucher NJ (1997) (S)NO signals Translocation, regulation, and a consensus motif. Neuron 18 691-6... 

GXSXG lipase consensus sequence 8 an-kyrin repeats Ca2+-in-dependent catalytic activity, proline-rich consensus motif (PX5PX8HHPX12NX4Q), LH-iPLA 2 (long isoform, 88 kDa) but not SH-iPLA2 (short isoform, 85 kDa) is activated by ATP GDSXV modified consensus sequence of serine esterase family catalytic triad Ser-His-Asp P and y subunits form a heter-otrimer with a 45-kDa noncatalytic subunit Ca2+-indepen-dent catalytic activity 0 Ca2+-independent catalytic activity... 

FIGURE 21.3 Action of class I and II cocaine-displaceable aptamers The effect of cocaine-displaceable aptamers 1-14 and II-3 on carbamoylcholine (lOOpM)-induced stimulation of acetylcholine receptor activity in the absence (control) and presence of cocaine was determined in vitro by fast kinetic electrophysiology. Aptamer 1-14 (0.5 pM) inhibited receptor activity (left panel) aptamer II-3 (5 pM) does not inhibit receptor activity (middle panel) aptamer II-3 (3 pM) alleviates inhibition of receptor function by cocaine (150 pM) (right panel). Prediction of the secondary structure [26] revealed the localization of consensus motifs of class-I and -II aptamers in stem-loop regions (letters in boxes). These stem loops are believed to be necessary for aptamer action. The figure reveals the data published in references [18, 19, 74]. 

A delineated class of DNA, minisatellite DNA, has been found to be moderately to extremely variable in certain eukaryote genomes.1-3 Minisatellite DNA is composed of tandem repeats of a core or consensus sequence reiterated a low to moderate number of times (relative to satellite DNA, where consensus motifs, or variants thereon, may be repeated tens of thousands of times). For convenience, minisatellite DNA as defined here includes simple sequence4 and microsatellite5 DNA. Thus, minisatellite consensus sequences range from 2 to approximately 70 base pairs (bp). Several different minisatellite families (members of a family have consensus sequence similarities) have been described.6... 

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