Conditional knockouts

Most importantly, later genetic experiments, using conditional knockout technique to delete all forms of CREB in either the entire forebrain or hippocampus, showed that these conditional knockout mice have normal hippocampal early- and late-phase LTP, and normal performance in hippocampal-dependent memory tasks [13]. Therefore, those newer findings cast significant doubt on the role of CREB as a central switch for long-term plasticity. 

Nelson We are looking at SK channels, and we have SK3 -overexpressing mice. These mice have difficulty giving birth because these SK3 channels are overexpressed in the uterus, and the smooth muscle can t contract properly. We have to suppress this expression (it s a conditional knockout) in order to keep our colony going. 

BDNF. x- Conditional knockout Constitutive knockout Rios etal. 2001 Lyons et al. 1999... 

To further dissect CRH/CRHRl central nervous system pathways modulating behavior from those regulating neuroendocrine function, a region-specific, conditional knockout mouse line CaMKII Cre) was generated... 

To summarize, knockouts have proved useful in determining the function of some genes. However, the complexity of gene interactions often leads to the absence of any phenotype, and multiple knockouts are required to tease apart the actual function of a gene or gene family. Moreover, certain genes are absolutely required during early development. Conditional knockout techniques have been developed in attempts to res-... 

South, S., Bogulavsky, J., Franklin, S., Martin, D., Vissel, B., Sailer, A., Malkmus, S., Masuyama, T., Horner, P., Gage, F., Tonegawa, S., Yaksh, T., Heinemann, S., Inturrisi, C. E. A conditional knockout (KO) of the NMDAR1 subunit in spinal cord dorsal horn attenuates hyperalgesia, Abstr. Society of Neurosciences Meeting, 2001. 

Integrin betal null platelets from conditional knockout mice develop normally, platelet count is normal. Collagen induced platelet aggregation is delayed but otherwise normal tyrosine phosphorylation pattern is normal but phosphorylation is delayed. Bleeding time in bone marrow chimeric mice is normal no major in vivo defects (Nieswandt et al. 2001). 

Shen TL, Park AY, Alcaraz A, Peng X, Jang I, Koni P, Flavell RA, Gu H, Guan JL. Conditional knockout of focal adhesion kinase in endothelial cells reveals its role in angiogenesis... 

Rojek A, Fuchtbauer EM, Kwon TH, Frokiaer J, Nielsen S Severe urinary concentrating defect in renal collecting duct-selective AQP2 conditional-knockout mice. Proceedings of the National Academy of Sciences of the United States of America 103 6037-6042,2006... 

Chapman, R. S., Lourenco, P. C., Tonner, E., Flint, D. J., Seibert, S.,Takeda, K., et al. (1999) Suppression of epithelial apoptosis and delayed mammary gland involution in mice with a conditional knockout of Stat3. Genes Dev. 13, 2604-2616. 

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