Concanavalin material

Combined scanning probe techniques 932 Compartmentalized surfaces 921 Competitive immunoassay el80 Composite electrodes 145 material 916 Concanavalin A 313, 317 Conducting polymer 44, 73 based ISE 74 Conductivity e62 Conductometric transducers 241 Conjugate 650 polymer 74... 

The selective precipitation of some polysaccharides with antisera, which has been studied by Heidelberger and coworkers, is an important analytical tool, but the purification of preparative amounts of material by this method seems to be impractical. This could also be said of precipitation with the globulin from Jack-bean meal, concanavalin A, which has been used by Smith and his coworkers for fractionation. ... 

Fig. 11.4. Laser confocal and SEM of the materials prepared according to the process depicted in Fig. 11.3. Photographs show individual stages in the cell-mediated lithography of polymer surfaces using Listeria monocytogenes (left column a, c, e, g) and Staphylococcus aureus (right column b, d, f, h) as templates. Imprinted microcapsules (a, b) and solid polymer beads before (c, d) and after (e, f) the removal of template cells, (g, h) Show the imprint sites after reacting the beads with fluorescent-labelled Concanavalin A.

One type of affinity-chromatography material of particular interest in carbohydrate chemistry is the insolubilized derivative of concanavalin A, a phytohemagglutin that shows specificity towards certain carbohydrates. 

Potent natriuretic activity was evident in a simple phosphate buffered saline extract of whole atria. 4 Subsequently, the material was found to be acid stable.76 7,88,100,101 while the extract is both acid and heat76.101 stable, trypsin,75,89,93 as well as other proteolytic enzymes76 such as chymotrypsin, ami nopeptidase A and carboxypeptidases B and C destroy natriuretic activity. However, treatment with carboxy-peptidase A only blunted activity,76 and concanavalin A had no effect.75 These observations are consistent with ANF being a small peptide. 

Fractions containing DOC-soluble glycopeptide obtained from the column of Bio-Gel A-5m were applied to a column of concanavalin A-Sepharose 4B and the column was washed with 40 Tris-HCl-40 Ti NaCl-0.24% DOC until the UV-absorbing material was removed. The DOC-soluble glycopeptide was eluted with 40 Tris-HCl-40 Ti NaCl-0.24% DOC-1% methyl-a-D-mannopyranoside. 

Table 1 Distribution of acidic and complex glycoforms of FSH during the ovarian cycle. Acidic glycoforms are determined as variants with pi <4.3 and complex glycoforms as non-retained material from Concanavalin A chromatography. Adapted from Ahobile eta/., 1998. ...

Hydrophilic urethane prepolymers are convenient materials for entrapment of enzymes jS-D-fructofuranosidase has been successfully immobilized in such a matrix. Immobilization of the same enzyme on a number of support matrices (cellulose, ionically charged dextrans, concanavalin A) has provided evidence for the involvement of salt links in its stabilization, as shown from pH activity curves, K values, and stability measurements. a-L-Fucosidase has been immobilized on concanavalin A-derivatized agarose and the effects of immobilization on pH dependence and thermal stability investigated. The enzyme was very strongly adsorbed to the matrix and its complete removal even under harsh elution conditions was not possible. 

Anderson, A.J., Green, R.S., Sturman, A.J. and Archibald, A.R. Cell Wall Assembly in Bacillus subtilis. Location of Wall Material Incorporated During Pulsed Release of Phosphate Limitation, its Accessibility to Bacteriophages and Concanavalin A, and its Susceptibility to Turnover , Journal of Bacteriology (191S), 136, 886-99... 

Sephadex (a dextran cross-linked with epichlorohydrin) has been used as an affinity matrix for the separation of [ I]-concanavalin A from the unlabelled lectin. If this technique is successful with other I-labelled proteins, it would help to overcome the uncertainty of whether or not biological properties displayed by labelled materials arise from contamination by the unlabelled material. 

The vibrational spectra of reference material are introduced in Figure 3.1, which belong to the main components of soft tissue. The IR spectrum (trace A) and Raman spectrum (F) of the all-beta protein concanavalin A are shown in Figure 3.1. IR bands due to the peptide backbone with P-sheet secondary structures are found at 3284 (amide A), 1636 (amide I), 1531 (amide II), and 1235 cm-i (amide III). Bands at 1403 (COO ) and 2963, 2874, 1455 cm- (CHg) are assigned to amino acid side chains. These bands are located in the Raman spectrum at similar positions at 1398 and 1449 cm . The Raman amide I band is centered at 1672 cm , the amide III band at 1238 cm , and the weak amide II band is not observed. Instead, other Raman bands of amino acids are identified at 759 and 1555 cm for Trp 621,1003, 1031, and 1208 cm for Phe 643, 829, and 853 cm for Tyr and 1126, 1317, and 1340 cm (CH2/CH3) for aliphatic amino acids. The IR spectrum (B) and Raman spectrum (G) of the all alpha protein bovine serum albumin show a number of differences. The amide bands... 

Concanavalin A (Con A) is a well known affinity protein-ligand for certain caibohydrates (Chapter 8 discusses the use of such support materials in detail). This affinity has been exploited in the resolution of closely related sugars on silica-bound Con A (18). Figure 8 demonstrates that a mixture of the three sugars p-nitrophenyl-/8-D-glucoside, p-nitro-phenyl-a-D-glucoside and p-nitrophenyl-a-o-maimoside, is resolved into three distinctive peaks in less than 10 min on a Con A-silica column. 

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