Enzymes compatible

Bioconjugates of Compatible Enzymes as Functional Catalysts for Multistep Processes... 

A key requirement for the successful on-line coupling of enzyme assays to ESI-MS is the solvent and buffer compatibility. Enzyme assays are mostly performed in nonvolatile buffers, such as HEPES, TRIS, and PBS. Moreover, additives are... 

Figure V-17 Cloning genes using a single or compatible enzymes (BamHUBglW).

NotI,(EagI),(Eael),MluI,(BssHII),XmaI,Sail,(Xhol) Recleavage enzymes BamHI, Xhol and compatible enzymes... 

BamHI,HindIII,XhoI and compatible enzymes (Enzymes in parentheses give compatible ends). 

Evaluate food-compatible enzyme inhibitors, such as citric and sulfur amino acids, and substrate inhibitors for their ability to inactivate SGT and other enzymes which catalyze glycoalkaloid biosynthesis (32). 

The next generation of amperomethc enzyme electrodes may weU be based on immobilization techniques that are compatible with microelectronic mass-production processes and are easy to miniaturize (42). Integration of enzymes and mediators simultaneously should improve the electron-transfer pathway from the active site of the enzyme to the electrode. 

These systems are allowed in many food appHcations, but there also exists a range of nonfood preservatives active over a broad pH range. However, these may not be compatible with all enzymes because of their inhibitory or denaturing effects. A usefiil reference on this subject is available (31). 

Bacterial a-amylases used in laundry detergents are fully compatible with detergent proteases, ie, the two enzymes work together in the wash process. During storage in both powder and Hquid detergents, the amylases are very stable in the presence of proteases. 

These results are compatible with an evolutionary history in which the new enzyme activity of mandelate racemase has evolved from a preexisting enzyme that catalyzes the basic chemical reaction of proton abstraction and formation of an intermediate. Subsequent mutations have modified the... 

Enzyme Common Isoschizomers Recognition Sequence Compatible Cohesive Ends... 

For an efficient enzymatic DKR the following requirements must be fulfilled (i) the KR must be very selective ( > 20) (ii) the racemization must be fast (at least 10 times faster than the enzyme-catalyzed transformation of the slow reacting enantiomer, krac >10 kent-s) (hi) the racemization catalyst must not react with the product of the reaction (iv) the KR and the racemization must be compatible under the same reaction conditions. 

Bowlus, R.D. Somero, G.N. (1979). Solute compatibility with enzyme function and structure rationales for the selection of osmotic agents and end products of anaerobic metabolism in marine invertebrates. Journal of Experimental Zoology, 208, 137-52. 

Careful empirical selection of the expression platform for carotenogenesis has included selection of the best strains for stability and degree of accumulation and the selection of compatible drug-resistance combinations and low copy number polycistronic plasmids to enhance product accumulation by decrease of metabolic burden." 5 Matthews and Wurtzel discussed culture and induction conditions - that have been explored in most studies. Most efforts to engineer carotenoid biosynthesis in E. coli focused on the genes and enzymes of the pathway and had a modest effect on improved accumulation. For example, substitution and over-expression of a GGPPS that uses IPP directly (discussed in... 

The lipase-catalyzed DKRs provide only (/ )-products to obtain (5 )-products, we needed a complementary (5 )-stereoselective enzyme. A survey of (5 )-selective enzymes compatible to use in DKR at room temperature revealed that subtilisin is a worthy candidate, but its commercial form was not applicable to DKR due to its low enzyme activity and instability. However, we succeeded in enhancing its activity by treating it with a surfactant before use. At room temperature DKR with subtilisin and ruthenium catalyst 5, trifluoroethyl butanoate was employed as an acylating agent and the (5 )-products were obtained in good yields and high optical purities (Table 3)P... 

Buffer The PCR buffer is usually provided as a 10-fold solution and is designed to be compatible with the enzyme. Common buffer components are 500 mM KCl 100 mM Tris-HCl, pH 9.3 1-2% Triton X-100 0.1% Tween. 

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