Comparative immunological methods

For over 25 years, the microcomplement fixation method1 has allowed rapid estimation of the approximate degree of sequence difference between monomeric, globular proteins from many different species.2,3 With this immunological method and two other ways of comparing proteins, elec-... 

Extraction methods and analytical procedures, mostly gas chromatography/mass spectrometry (GC/MS), are described in other chapters of this book, and have been extensively compared in recent years. It has been shown that immunological methods... 

An immunological method has been developed based on AFBj antibody-functionalised composite magnetic nano particles based on FejO SiO. For this purpose anti-AFBj is immobilized covalently on the surface of the nucleus. Te selectivity, reproducibility and stability of the immunological system are within the allowable error. Compared to the conventional ELISA the proposed immunosystem is simple and rapid (Li Wang and Xian-Xue Gan 2009). 

The Enzymic-Immunologic Method in Clinical and Forensic Toxicology. Comparative Analysis in Diphenyl-hydantoin Assays Carried Out with EMIT [Enzyme-Multiplied Immunoassay Technique] and Gas Chromatography... 

An extremely important point in process characterization is validation with, e.g., classical methods, such as gas chromatography (GC) Fig. 14) or liquid chromatography. Atrazine in surface waters was measured by means of ELISA and GC, and the results compared. The results correlate well, and confirm the theory that immunological methods can give reliable values in practice. The results of both methods are equivalent with a probability of 95 %, No false negative values were obtained. The same applies to the use of immunoassays on soil extracts. In comparison with GC analyses, ELISA usually gives higher analyte concentrations, which is put down to the effects of humic acid. 

Indeed, recently we developed an enzymatic bioluminescent assay for SBA (21) with a sensitivity comparable to that of immunological methods. 

Biopolymers are employed in many immunological techniques, including the analysis of food, clinical samples, pesticides, and in other areas of analytical chemistry. Immunoassays (qv) are specific, sensitive, relatively easy to perform, and usually inexpensive. For repetitive analyses, immunoassays compare very favorably with many conventional methods in terms of both sensitivity and limits of detection. 

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