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Columns, reversed HPLC dimensions

In reversed-phase HPLC, the E vitamers are eluted in the order <5-T-3, y-T-3, y3-T-3 (unresolved), a-T-3, <5-T, y-T, (3-T (unresolved), and a-T. This elution profile contrasts with that obtained using normal-phase chromatography, in which a-T is eluted first and 5-T-3 is eluted last. The positional (3 and y isomers of tocopherols and tocotrienols cannot be separated using reversed-phase columns of standard dimensions, even if gradient elution is used. a-Tocopheryl acetate elutes immediately in front of a-tocopherol, with baseline separation. [Pg.380]

HPLC has been used in the extraction and purification of pollutants in environmental and biological samples. Its manifold applications are due to the availability of a number of stationary phases, particularly reversed phase columns of various dimensions. HPLC instruments used for this purpose are similar to the analytical machine but with the use of a different column, which depends on the nature of the pollutants to be extracted and purified. Many of the reports available in the literature deal with the use of HPLC as an extraction and purification technique for environmental and biological samples. For determinations of polar pesticides, HPLC appears to be the most appropriate technique for purification purposes [134, 135]. Smith etal. [136] used low pressure HPLC for the extraction of polychlorinated dibenzofurans and dioxins. Furthermore, Blanch era/. [137] used low pressure HPLC for the purification and extraction of PCBs from shark liver. Similarly, Ramos etal. [138] used the Smith etal. [136] method for the extraction of PCBs in dolphin liver. Bethan etal. [139, 140] used a LiChrosorb 100-Si HPLC column for the extraction of bromocyclen collected from river water and of ck-HCH in marine water. However, HPLC cannot achieve a reputation as the universal and highly applicable extraction technique, due to its low range with regard to preparative chromatography. Therefore, further advancement of the technique is required, especially with regard to the development of preparative columns. [Pg.170]

A six-port valve was first used to interface the SEC microcolumn to the CZE capillary in a valve-loop design. UV-VIS detection was employed in this experiment. The overall run time was 2 h, with the CZE runs requiring 9 min. As in the reverse phase HPLC-CZE technique, runs were overlapped in the second dimension to reduce the apparent run time. The main disadvantage of this yu-SEC-CZE method was the valve that was used for interfacing. The six-port valve contributed a substantial extracolumn volume, and required a fixed volume of 900 nL of effluent from the chromatographic column for each CZE run. The large fixed volume imposed restrictions on the operating conditions of both of the separation methods. Specifically, to fill the 900 nL volume, the SEC flow rate had to be far above the optimum level and therefore the SEC efficiency was decreased (22). [Pg.206]

The combination of normal (silica) and reversed (C18) phase HPLC in a comprehensive 2D LC system was used for the first time for the analysis of alcohol ethoxylates [64] the NP separation was run using aqueous solvents, so the mobile phases used in the two dimensions were miscible, resulting in the easy injection of the entire first-dimension effluent onto the second-dimension column. [Pg.112]

A sensitive reverse-phase HPLC method has been developed for the analysis of etodolac in tablet formulation [22]. The chromatographic separation was achieved using a reverse-phase Cu column, having dimensions of 3.3 cm x 0.46 cm i.d. (3 pm particles) and which was maintained at 30°C. The mobile phase consisted of pH 6.0 phosphate buffer / methanol (60 40 v/v), and was eluted at 1 mL/min. Analyte detection was effected on the basis of UV detection at 230 nm. Diazepam was used as an internal standard. The sample preparation entailed grinding the etodolac tablets, followed by extraction with methanol (using sonication). A retention time of 1.46 min was obtained for etodolac under these conditions, and the method was found to be linear, precise, and accurate over the concentration range of 0.01 to 0.1 mg/mL. [Pg.132]

Reverse phase high pressure liquid chromatography (HPLC) was used to further fractionate the sample and add another dimension of specificity (8,21). The extract was evaported to dryness and taken up in CHCI3. The entire extract was injected onto a DuPont Zorbax ODS column at 40°C using 2 cc/min. CH3OH mobile phase. Typical chromatograms are shown in Figure 2. [Pg.280]

Figure 13.3. Two-dimensional map showing the LC-MS analysis of an extract of red clover under investigation for the management of menopause. Reversed phase separation was carried out using a Ci8 HPLC column in the time dimension and negative ion electrospray mass spectrometry was used for compound detection and molecular weight determination in the second dimension. Figure 13.3. Two-dimensional map showing the LC-MS analysis of an extract of red clover under investigation for the management of menopause. Reversed phase separation was carried out using a Ci8 HPLC column in the time dimension and negative ion electrospray mass spectrometry was used for compound detection and molecular weight determination in the second dimension.
Although liquid chromatography techniques have become quite popular in the separation of peptides in complex protein digests, they are yet to make an impact for the separation of protein samples for proteome-wide applications. It is envisioned that in the future their application for protein separation will increase. Various combinations of reversed-phase (RP)-HPLC with ion-exchange, size-exclusion, chromato-focusing (CF), IEF, and capillary electrophoresis (CE) have emerged for 2D separation of complex mixtures of proteins and peptides. A recent addition in this field is the use of CF as the first dimension and RP-FIPLC as the second-dimension separation device.14 CF is a column-based liquid-phase separation technique, in which proteins are fractionated on the basis of differences in their p/values in a weak ion-exchange column. [Pg.462]

Successful enantioseparation of individual N -protected amino acids stimulated the development of a rapid method of their simultaneous enantioseparation and quantification in a mixture. A feasibility study on this topic has been recently published by Welsch et al. [69]. The two-dimensional HPLC method involves online coupling of a narrow-bore C18 reverse phase (RP) column in the first dimension (separation of racemic amino acids) to a short enantioselective column based on nonporous 1.5 pm particles modified with t-BuCQD in the second dimension (determination of enantiomer composition). Using narrow-bore column resulted in fast analysis time for example, the mixture of nine racemic N-DNB-protected amino acids was completely analyzed within 16 min. [Pg.437]

Figure 5.4-4. Scheme of an offline MDLC setnp. The separation with this two-dimensional HPLC separation is divided into two steps. At the first step, the analyte is separated by an ion-exchange separation (lEX) and the eluted fractions are collected. Before the second step, the samples are concentrated and desalted and organic modifiers are removed if present. The second dimension is performed by a normal reversed phase-HPLC separation (see Figure 5.4-1). If the required capacities are not available, this type of MDLC run enables the separation of both dimensions on the same HPLC after a setup change of column and solvents. Figure 5.4-4. Scheme of an offline MDLC setnp. The separation with this two-dimensional HPLC separation is divided into two steps. At the first step, the analyte is separated by an ion-exchange separation (lEX) and the eluted fractions are collected. Before the second step, the samples are concentrated and desalted and organic modifiers are removed if present. The second dimension is performed by a normal reversed phase-HPLC separation (see Figure 5.4-1). If the required capacities are not available, this type of MDLC run enables the separation of both dimensions on the same HPLC after a setup change of column and solvents.
The quality of the separation depends, among other things, on the steepness of the gradient and the temperature. The temperature is in play because peptides can maintain a secondary structure (a-helix, P-fold) imder the conditions of the reversed-phase HPLC, which influences the adsorption. High temperatures prevent secondary structures. Because it is more comfortable, people predominantly work at RT. Regarding the column dimensions the separation of peptides and smaller proteins improves with longer columns. For tryptic digestion, people... [Pg.113]


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Column dimensions

Columns, reversed HPLC

HPLC column

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