Column-packing technology

Both Abcor and Elf-SRTF have developed gas chromatographic processes for the separation of a and ji pinenes. The processes, which are similar in general principle and differ mainly in the methods used to pack the column, are compared in Table 10.1. By improvements in column-packing technology Elf-SRTI were able to eliminate baffles and still achieve a reasonably low HETP in a production scale column. (// 1.0 mm for a 40 cm. diameter column.) However, even in the largest of these units throughput is only about... 

Modem technologies provide many techniques for expanding the throughput of an analytical laboratory. The task that needs to be accomplished and the possible drawbacks should be carefully considered. Optimized LC equipment can utilize columns packed with much smaller stationary phase particles to achieve significant reductions in gradient time while still achieving the same or even better peak capacities than conventional methods. 

Since amino acids and nucleotides are all polar and hydrophilic, they will be eluted quickly by the column. The mobile phase (see below) is also selected on the basis of polarity, with a medium- to high-polarity solvent required. The opposite of reverse phase chromatography is normal phase, where the column packing is medium to high polarity and the mobile phase is nonpolar. This technology is generally not applied to the analysis of polar molecules such as amino acids or nucleotides. Some peptides are more hydrophobic, making this method potentially more useful for peptides than for amino acids or nucleotides. 

The influence of HPLC on the development of separation media for CEC is rather obvious. For example, HPLC-like hardware , such as frits and packed columns, are employed. A number of various packing technologies have been reported that enable packing particles into narrow bore capillary columns. The solvent slurry packing appears to be the most popular technique that has been transferred di-... 

Thermodynamic behaviors and retention mechanisms for SFC are unique. Low temperatures and high pressures or high densities usually favor fast separation of enantiomers in SFC. In the case that the isoelution temperature is below the working temperature, the selectivity increases as temperature increases and higher temperatures are favorable for chiral separation. Future development in SFC will likely include new chiral column technologies and instrumentation refinement. A greater variety of chiral columns packed with smaller particles will open up more areas of application for fast chiral separations. In addition, improvement in signal-to-noise ratio of... 

For reasons that will be explained in a subsequent chapter, the team found that the application of a technology known as bioflitration was considered the best remediation method. The contaminated gas is pumped through a column packed with a material that performs two functions (1) it provides for efficient contact between the air passing through it and water, and (2) it serves as a scaffold for the development of a bacterial colony. Figure 1.7 shows a typical biofiltration arrangement. The team must develop a system that has a very low pressure drop so that the process can be constructed from plastic tanks and low-pressure pumps to save money. In a further effort to save money, the process should have a small footprint. 

Correct column packing is obtained by using preparative columns equipped with dynamic axial compression (DAC) technology [10, 11]. The column includes a movable piston attached to a hydraulic jack. The piston is used to pack and unpack the column and to maintain the stationary phase under dynamic compression, ensuring perfect particle stacking (Figure 12.5) and bed stability through time. 

The advances in column and instrument technology have significantly enhanced HPLC performance in recent years. Results comparing the effects of various column packings on TG separation by RP-HPLC were presented by El-Hamdy and Perkins (87). Six commercially packed columns produced by different manufacturers were used PARTISIL ODS-1 and ODS-2 octadecyl-bonded silica of 10-/rm partical size, ZORBAK-ODS octadecyl-silica of 6-7-/rm diameter (250 X 4.6-mm ID), 5-/rm octyl-bonded spherical silica LC-8, 5-//m methyl-bonded spherical silica LC-1, and a 5-/rm octadecyl-bonded spherical silica LC-18 (150 X 4.6-mm ID). The mobile phase employed consisted of mixtures of methanol/acetone/isopropanol/acetonitrile ranging from l 0 3 4to 1 6 3 4. Triglycerides were solubilized in either THF or acetone at 100 mg/ml for each compound. 

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